高等学校化学学报

高等学校化学学报 ›› 1993, Vol. 14 ›› Issue (12): 1653.

• 研究论文 • 上一篇    下一篇

反相离子对液相色谱分离-柱后衍生化学发光检测痕量金属离子

张新祥, 程介克, 曾云鹗   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1992-12-11 修回日期:1993-07-20 出版日期:1993-12-24 发布日期:1993-12-24
  • 通讯作者: 程介克
  • 作者简介:张新祥, 男, 30岁, 博士后.
  • 基金资助:

    国家自然科学基金

Determinationn of Trace Amount of Metal Ions by Reversed-Phase Ion-Pair Liquid Chromatography with Post-Column Derivatization Chemiluminescence Detection

ZHANG Xin-Xiang, CHENG Jie-Ke, ZENG Yun-E   

  1. Department of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1992-12-11 Revised:1993-07-20 Online:1993-12-24 Published:1993-12-24

PDF (PC)

被引次数

22

摘要： 报道了反相离子对液相色谱-柱后衍生化学发光检测方法分离测定痕量的钴(Ⅱ)、铁(Ⅱ)、铜(Ⅱ)、铁(Ⅲ)及铬(Ⅲ).利用弱络合性的乳酸作为淋洗剂,加入适量的十二烷基磷酸钠作对离子试剂,研究了分离条件、发光条件及二者的匹配方式等因素对分离检测的影响.测定线性范围分别为(g/mL):Cr(Ⅲ)5.0×10-9～1.0×10-5;Fe(Ⅱ)2.0×10-9～1.0×10-5;Fe(Ⅲ〕8.0×10-9～1.0×10-5;Cu(Ⅱ)8.0×10-11～1.0×10-5及Co(Ⅲ)1.0×10-11～1.0×10-5.检出限分别为:10pgCr(Ⅲ),0.4pgFe(Ⅱ),20pgFe(Ⅲ),1.0pgCu(Ⅱ)及0.01pgCo(Ⅱ).

关键词: 反相离子对液相色谱, 柱后衍生化学发光测定, 金属离子

Abstract: The separation and determination of Cr(Ⅲ), Fe(Ⅱ), Fe(Ⅲ), Cu(Ⅱ), and Co(Ⅱ) were demonstrated by reversed-phase ion-pair liquid chromatography with chemiluminescence detection.Areversed-phase column (YWQ-C18, 10 μm, 150×4.0 mm i.d.) was used.The ion-pair ion was sodium dodecyl sulfate (SDS) and the eluent was lactic acid.The Chemiluminescence system used was luminol-KOH-H2O2-Brij 35 system.The separation and detection conditions were studied.In the optimization condition the metal ions could be determined in the range from 1.0×10-11 to 1.0×10-5g/mLwith the detection limits of 10 pg Cr(Ⅲ), 0.4 pg Fe (Ⅱ), 20 pg Fe(Ⅲ), 1.0 pg of Cu(Ⅱ), and 0.01 pg Co(Ⅱ), respectively.

Key words: Reversed-phase ion-pair liquid chromatography, Post-column derivatization chemilu-minescence detection, Metal ions

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