高等学校化学学报

高等学校化学学报 ›› 1989, Vol. 10 ›› Issue (6): 638.

• 研究论文 • 上一篇    下一篇

多孔固相放射免疫测定载体的合成和应用研究

由英才1, 梁逊1, 滕欣莲1, 郭贤权1, 侯秉璋2, 尹伯元2   

  1. 1. 南开大学化学系;
    2. 天津医学院同位素研究室
  • 收稿日期:1987-11-14 出版日期:1989-06-24 发布日期:1989-06-24
  • 通讯作者: 由英才

Studies on Synthesis and Application of Porous Carrier for Solid Phase Radioimmunoassay

You Yingcai1, Liang Xun1, Teng Xinlian1, Guo Xianquan1, Hou Bingzhang2, Yin Boyuan2   

  1. 1. Department of Chemistry, Nankai University, Tianjin;
    2. Isotope Institute, Tianjin Medical College, Tianjin
  • Received:1987-11-14 Online:1989-06-24 Published:1989-06-24

PDF (PC)

被引次数

1

摘要： 合成了一种具有交联结构的多孔性甲状腺素(T4)固相放射免疫测定载体微球,并对载体微球的功能基转化、悬臂的引入和功能基的活化反应进行了研究。用合成的载体微球与甲状腺素抗体偶联,通过测定甲状腺素的实验证明,该微球具有较高的结合率。

关键词: 甲状腺素(T4), 固相放射免疫测定, 微球, 致孔剂

Abstract: Porous microspheres with diameter ranging from 250 to 400 mesh and pore sizes from 1524 to 4304Å were synthesized by suspension polymerization of MMAand DVBwith 26-50% paraffin liquid or 30-50% 200# petrol by weight as porogenic agents. The microspheres extracted with petroleum ether were hydrolyzed (50% NaOH, 140-150℃; 1-5 h). The microspheres with spacer were prepared by reacting carboxylated microspheres with 1,6-hexamethylene diamine. The T4 -anti-body was covalently coupled to the microspheres with spacer by a two-step glutaraldehyde method. The reactivity of the microsphere-anti-body conjugates was characterized by reacting the microsphere-anti-body conjugates with 125Ilabeled thyroxine, the binding percentage is 94%.The results of our experiments showed that the porous microspheres is a ideal carrier for solid phase radioimmunoassay of thyroxine.

Key words: Thyroxine(T4), Solid phase radioimmunoassay, Microsphere, Poroge- nic agents

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