高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

DNA链二级结构调控的分子信号变换

李子慕1,汤雨晴1,程嘉宁1,孙晨蕴1,吕慧2,左小磊1   

  1. 1.上海交通大学化学化工学院化学生物协同物质创制全国重点实验室，新基石科学实验室，变革性分子前沿科学中心，转化医学国家科学中心
    2.上海大学理学院材料生物学研究所
  • 收稿日期:2025-06-25 修回日期:2025-07-20 网络首发:2025-08-12 发布日期:2025-08-12
  • 通讯作者: 左小磊 E-mail:zuoxiaolei@sjtu.edu.cn
  • 基金资助:
    国家自然科学基金（批准号：T2188102）资助

Molecular signal transformation via secondary structure modulation of DNA strands

LI Zimu1, TANG Yuqing1, CHENG Jianing1, SUN Chenyun1, LV Hui2, ZUO Xiaolei1   

  1. 1. State Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules, National Center for Translational Medicine, Shanghai Jiao Tong University
    2. Institute of Materiobiology, College of Sciences, Shanghai University
  • Received:2025-06-25 Revised:2025-07-20 Online First:2025-08-12 Published:2025-08-12
  • Contact: ZUO Xiaolei E-mail:zuoxiaolei@sjtu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China (No. T2188102 )

PDF (PC)

摘要： 本文对DNA置换反应的输入单链引入多种不同类型的自身互补结构，以改变其自由能及构象，探究不同结构对链置换反应速率的影响.我们发现，链置换反应速率随互补结构长度的增加而减慢.这一现象在立足点域设计互补结构的效果比干区形成互补结构更为明显. 在链置换反应的输入链中引入二级结构，对DNA链置换反应速率产生不同程度的降低，甚至阻止反应进行.最后，本文阐释了二级结构影响链置换反应速率的机制，为扩宽DNA链置换反应的时域调控能力，并提升其DNA分子计算、生物传感等领域的提供了新思路.

关键词: DNA链置换反应, 二级结构, 反应动力学

Abstract: In this study, various types of self-complementary secondary structures were introduced into the input single strand DNA of strand displacement reactions to change their free energy and conformations, aiming to investigate the influence of different structures on the rate of strand displacement reaction. We found that the reaction rate decreased as the length of the self-complementary structure increased. This effect was more pronounced when the self-complementary structure was designed in the toehold domain than when it formed in the non-toehold region. The incorporation of secondary structures into the input strands led to varying degrees of reduction in strand displacement reaction rates, and in some cases, completely inhibited the reaction. Finally, we elucidate the mechanism by which secondary structures influence the kinetics of strand displacement reactions, offering new insights into expanding the temporal regulation capabilities of DNA strand displacement and advancing its applications in DNA molecular computing and biosensing.

Key words: DNA strand displacement reaction, Secondary structure, Reaction kinetics

中图分类号: 

TrendMD: