高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (3): 455-462.doi: 10.7503/cjcu20170328

• 分析化学 • 上一篇    下一篇

基于SBA-15的表面增强拉曼基底的制备及对鸡肉和鸡饲料中恩诺沙星的检测

孙琳, 张涵, 杜一平()   

  1. 华东理工大学化学与分子工程学院, 上海市功能性材料化学重点实验室, 上海 200237
  • 收稿日期:2017-05-26 出版日期:2018-03-10 发布日期:2018-01-02
  • 作者简介:联系人简介: 杜一平, 男, 博士, 教授, 主要从事分子光谱及化学计量学研究. E-mail:yipingdu@ecust.edu.cn
  • 基金资助:
    中央高校基本科研业务费专业资金(批准号: 222201714047)资助

Preparation of Surface Enhanced Raman Scattering Substrates Based on SBA-15 Material and the Detection of Enrofloxacin in Chicken and Chicken Feed

SUN Lin, ZHANG Han, DU Yiping*()   

  1. Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2017-05-26 Online:2018-03-10 Published:2018-01-02
  • Contact: DU Yiping E-mail:yipingdu@ecust.edu.cn

摘要:

在氨基改性的多孔材料SBA-15表面修饰金纳米颗粒制备了表面增强拉曼(SERS)基底, 以四巯基吡啶(4-Mpy)作为探针分子, 对基底的SERS性能进行评价. 结果显示, 利用金纳米粒子在介孔材料中的有效负载形成的结构有利于SERS信号的增强, 基底在保存5个月后信号降低幅度较小, 稳定性好. 将此基底应用于鸡肉和鸡饲料提取液中恩诺沙星的SERS检测, 在0.1~1 mg/kg浓度范围内, 特征峰的拉曼信号强度与恩诺沙星浓度具有良好线性关系(R2分别等于0.98和0.99), 检测限均达到0.1 mg/kg. 该检测方法简单快速、 灵敏度高、 稳定性好, 为SERS技术应用于抗生素的快速检测提供了新的途径.

关键词: 表面增强拉曼基底, 恩诺沙星, 快速检测, 介孔材料

Abstract:

A novel surface-enhanced raman scattering(SERS) substrate based on gold nanoparticles decorated amino group modified SBA-15(Au@NH2-SBA-15) was developed. The synthetic conditions of the substrate were optimized including the time of amino modification and the volume of gold colloid using 4-mercaptopyridine(4-Mpy) served as a molecular probe, and the SERS performance was evaluated. The results showed that based on effective loading gold nanoparticles, the developed SERS substrate exhibited stronger SERS intensities and better stability with small signal reduction within five months comparing with traditional gold colloid substrate. The substrate was applied to detect enrofloxacin in chicken and chicken feed. The linear relationship between concentration of enrofloxacin and Raman intensity was good in the range of 0.1—1 mg/kg(R2 equals 0.98 and 0.99 for the two samples), and the detection limit of enrofloxacin reached 100 μg/kg. The developed method with advantages of simpleness, rapidness, high sensitivity and good stability, contributed a favorable way to detect illegal additives of antibiotic with SERS.

Key words: Surface-enhanced Raman scattering(SERS) substrate, Enrofloxacin, Rapid detection, Mesoporous material

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