高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (4): 813-818.doi: 10.7503/cjcu20121098

• 分析化学 • 上一篇    下一篇

银杏叶中α-葡萄糖苷酶抑制剂的超滤质谱筛选

刘洋1, 周慧2, 刘舒2, 刘志强2   

  1. 1. 吉林大学化学学院, 长春 130012;
    2. 中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:2012-12-05 出版日期:2013-04-10 发布日期:2013-03-20
  • 通讯作者: 刘志强,男,博士,研究员,博士生导师,主要从事天然产物质谱及中药活性成分筛选方面的研究.E-mail:liuzq@ciac.jl.cn E-mail:liuzq@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号:21175128,21073178,81073040),吉林省自然科学基金(批准号:201015101)和吉林省科技发展计划重点项目(批准号:20110928)资助.

Screening of α-Glucosidase Inhibitors in Ginkgo Biloba Extract Using Ultrafiltration LC-ESI-MSn

LIU Yang1, ZHOU Hui2, LIU Shu2, LIU Zhi-Qiang2   

  1. 1. College of Chemistry, Jilin University, Changchun 130012, China;
    2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2012-12-05 Online:2013-04-10 Published:2013-03-20

摘要:

采用体外酶抑制活性检测方法结合超滤质谱(UF-LC/MS)筛选方法对中药提取物中的α-葡萄糖苷酶抑制剂进行了筛选. 以4-硝基苯-α-D-吡喃葡萄糖苷(PNPG)为底物, 阿卡波糖为阳性对照药, 对5种富含黄酮类化合物的中药提取物进行了α-葡萄糖苷酶抑制活性的初步测定. 结果表明, 银杏叶具有最强的α-葡萄糖苷酶抑制活性, 可作为进一步复筛的对象. 利用超滤质谱技术对银杏叶中潜在的α-葡萄糖苷酶抑制剂进行了筛选, 从中筛选出4种潜在的α-葡萄糖苷酶抑制剂, 并利用液相色谱-串联质谱技术(LC-MSn)对其结构进行了鉴定. 本文结果为开发新一代安全有效的降糖药物奠定了基础.

关键词: 银杏叶, α-葡萄糖苷酶抑制剂, 超滤质谱, 筛选

Abstract:

In vitro α-glucosidase inhibition assays and ultrafiltration liquid chromatography coupled to electrospray ionization tandem mass spectrometry(ultrafiltration LC-ESI-MSn) were combined to screen α-glucosidase inhibitors from ginkgo biloba extract. The enzyme inhibition studies were carried out spectrophotometrically in a 96-well microplate reader. Using 4-nitrophenyl α-D-glucopyranoside(PNPG) as the substrate and acarbose as a positive control, α-glucosidase inhibitory activity of the five kinds of Chinese herb extracts which rich in flavonoids were determined, the results showed that the ginkgo biloba extract has the strongest the α-glucosidase inhibitory activity. Ultrafiltration LC-ESI-MSn was used to screen and identify α-glucosidase inhibitors from ginkgo biloba extract. As a result, four compounds were identified as α-glucosidase inhibitors in the ginkgo biloba extract, and their structures were confirmed to be quercetin, apigenin, kaempferol and isorhamnetin by ESI-MS.

Key words: Ginkgo biloba, α-Glucosidase inhibitor, Ultrafiltration mass spectrometry, Screening

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