高等学校化学学报 ›› 2014, Vol. 35 ›› Issue (8): 1640-1645.doi: 10.7503/cjcu20140419

• 分析化学 • 上一篇    下一篇

离心超滤质谱法筛选中药复方二妙丸中黄嘌呤氧化酶抑制剂

徐晨1,2, 刘舒1(), 刘志强1, 宋凤瑞1   

  1. 1. 中国科学院长春应用化学研究所, 国家质谱中心(长春), 吉林省中药化学与质谱重点实验室, 长春 130022;
    2. 中国科学院大学, 北京 100049
  • 收稿日期:2014-05-04 出版日期:2014-08-10 发布日期:2019-08-01
  • 作者简介:联系人简介: 刘 舒, 女, 博士, 助理研究员, 主要从事中药活性成分筛选及作用机制研究. E-mail: mslab20@ciac.ac.cn
  • 基金资助:
    国家自然科学基金(批准号: 21175128, 81303280)资助

Screening of Xanthine Oxidase Inhibitors in Traditional Chinese Herbal Formulae Ermiaowan Using Ultrafiltration UPLC-MSn

XU Chen1,2, LIU Shu1,*(), LIU Zhiqiang1, SONG Fengrui1   

  1. 1. National Center for Mass Spectrometry in Changchun, Jilin Province Key Laboratory for Traditional Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2014-05-04 Online:2014-08-10 Published:2019-08-01
  • Contact: LIU Shu E-mail:mslab20@ciac.ac.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China(Nos.21175128, 81303280)

摘要:

采用离心超滤质谱分析技术(UF-UPLC/Q-TOF/MS), 结合体外酶活性实验方法, 对中药复方二妙丸提取物中的黄嘌呤氧化酶抑制剂进行了筛选. 体外酶活性实验测得二妙丸水提液对黄嘌呤氧化酶的半数抑制浓度(IC50)为(0.218±0.0034) mg/mL, 表明二妙丸具有较强的黄嘌呤氧化酶抑制活性. 进一步采用离心超滤质谱技术对二妙丸水提物中潜在的黄嘌呤氧化酶抑制剂进行筛选, 从中筛选并鉴定了9种具有潜在黄嘌呤氧化酶抑制活性的化合物, 为开发黄嘌呤氧化酶抑制剂及阐明二妙丸治疗痛风和高尿酸血症的作用机制提供了一定的依据.

关键词: 二妙丸, 黄嘌呤氧化酶, 离心超滤质谱

Abstract:

An ultrafiltration UPLC/Q-TOF/MS assay and in vitro xanthine oxidase inhibition assay were combined to screen xanthine oxidase inhibitors in traditional Chinese herbal formulae Ermiaowan. The result of in vitro xanthine oxidase inhibition assay showed that Ermiaowan had a strong xanthine oxidase inhibitory activity, the IC50 value was (0.218±0.0034) mg/mL. Nine compounds were screened and identified as potential xanthine oxidase inhibitors in Ermiaowan using ultrafiltration UPLC/Q-TOF/MS. This work provides a certain basis for the development of xanthine oxidase inhibitors and the mechanism clarification of Ermiaowan to treat gout and hyperuricemia.

Key words: Ermiaowan, Xanthine oxidase, Ultrafiltration UPLC/Q-TOF/MS

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