高等学校化学学报

高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (12): 2827.doi: 10.7503/cjcu20120006

• 高分子化学 • 上一篇    

静电纺丝法制备PBS纳米纤维膜及缓释血小板内生长因子的PBS纳米纤维膜的生物活性评价

孙淑芬1, 李男男1, 刘敏1, 刘译聪1, 张英超2, 周延民1   

  1. 1. 吉林大学口腔医院, 长春 130021;
    2. 吉林大学超分子结构与材料国家重点实验室, 长春 130012
  • 收稿日期:2012-01-04 出版日期:2012-12-10 发布日期:2012-11-20
  • 通讯作者: 周延民,男,博士,教授,博士生导师,主要从事种植义齿和骨代谢研究.E-mail:zhouym@jlu.edu.cn E-mail:zhouym@jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 81070855)资助.

Preparation and Bioactivities of Electrospun PBS Nanofiber Membrane and Hybrid PBS Nanofiber Membrane of Sustained Release Growth Factors in Platelet

SUN Shu-Fen1, LI Nan-Nan1, LIU Min1, LIU Yi-Cong1, ZHANG Ying-Chao2, ZHOU Yan-Min1   

  1. 1. Stomatological Hospital of Jilin University, Changchun 130021, China;
    2. State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, China
  • Received:2012-01-04 Online:2012-12-10 Published:2012-11-20
  • Contact: Yan-min ZHOU E-mail:zhouym@jlu.edu.cn

PDF (PC)

被引次数

9

摘要：

利用静电纺丝法制备了聚丁二酸丁二醇酯(PBS)纳米纤维膜及PBS和富血小板血浆(PRP)的混合纳米纤维膜. 通过扫描电子显微镜、 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法、 支架表面细胞荧光染色法、 材料溶血行为及Elisa法检测体外释放综合评价了支架材料的性能及生物活性. 结果表明, 纳米纤维膜对人骨肉瘤细胞系MG63细胞增殖具有促进作用; 生长因子在最初的突释后, 随材料降解而缓释; 支架材料溶血率为0.1475%(<5%), 符合医用材料的溶血实验要求.

关键词: 纳米纤维膜, 聚丁二酸丁二醇酯, 生长因子, 富血小板血浆, 缓释

Abstract:

Poly(butylene succinate)(PBS) nanofiber membrane and hybrid nanofiber membrane of PBS and platelet-rich plasma(PRP) were prepared by electrospinning. The properties and bioactivities of scaffold materials were evaluated by means of scanning electron microscope(SEM), methyl thiazolyl tetrazolium(MTT), fluorescent staining and laser scanning co-focal microscope, hemolytic properties of materials and enzyme-linked immunosorbent assay(ELISA). The experiment results show that nanofiber membranes can accelerate the proliferation of human osteosarcoma MG63 cells line. Also, in vitro the growth factors in the scaffold material display a sustained release with the scaffold degrading after the initial burst release. In addition, hemolytic rate of scaffold material is 0.1475%(<5%), which meets the demand of hemolytic rate of medial materials.

Key words: Nanofiber membrane, Poly(butylene succinate), Growth factor, Platelet-rich plasma, Sustained release

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