Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (10): 1470.

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Interaction of Proteins with Fe(Ⅲ)-Chrome Azurol S Complex

WEI Yong-Ju, LI Ke-An, TONG Shen-Yang   

  1. Department of Chemistry, Peking University, Beijing, 100871
  • Received:1993-11-22 Revised:1994-04-18 Online:1994-10-24 Published:1994-10-24

Abstract: In pH 3.0 solution, proteins may react with Fe(Ⅲ)-chrome azurol Sbinary complex to form stable ternary complexes.The maximum absorption wavelengths of the binary and ternary complexes are 570 nm and 660 nm, respectively.According to the absorption spectra of this system, we consider that the combination is primarily due to electrostatic forces between protonated ammonium group of proteins and sulfonic acid group and carboxyl group of chrome azurol S.The sensitiveties(Sandell index)of detection for bovine serum albumin and human γ-globulin are 0.04and 0.16μg/cm2, respectively, which are about 15 to 35 times as high as that of the bromocresol green method.

Key words: Serum albumin, γ-globulin.Fe(Ⅲ)-chrome azurol S complex, Absorption spectrum

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