Interaction of Proteins with Fe(Ⅲ)-Chrome Azurol S Complex

Abstract

Abstract: In pH 3.0 solution, proteins may react with Fe(Ⅲ)-chrome azurol Sbinary complex to form stable ternary complexes.The maximum absorption wavelengths of the binary and ternary complexes are 570 nm and 660 nm, respectively.According to the absorption spectra of this system, we consider that the combination is primarily due to electrostatic forces between protonated ammonium group of proteins and sulfonic acid group and carboxyl group of chrome azurol S.The sensitiveties(Sandell index)of detection for bovine serum albumin and human γ-globulin are 0.04and 0.16μg/cm², respectively, which are about 15 to 35 times as high as that of the bromocresol green method.

Key words: Serum albumin, γ-globulin.Fe(Ⅲ)-chrome azurol S complex, Absorption spectrum

TrendMD:

WEI Yong-Ju, LI Ke-An, TONG Shen-Yang. Interaction of Proteins with Fe(Ⅲ)-Chrome Azurol S Complex[J]. Chem. J. Chinese Universities, 1994, 15(10): 1470.

[1]	CHENG Xiao, K BORA Debajeet, GLANS Per⁃Anders, GUO Jinghua, LUO Yi. An In-depth Theoretical Study of Ligand Field and Charge Transfer Effects on Co²⁺_2pL_2，3-edges X-ray Absorption Spectra [J]. Chem. J. Chinese Universities, 2021, 42(7): 2197.
[2]	LI Mengshuo, ZHANG Jing, LIU Dan, ZHU Yaxian, ZHANG Yong. Interactions of Pyrene with Human Serum Albumin and Bovine Serum Albumin： Microenvironmental Polarity Differences at Binding Sites [J]. Chem. J. Chinese Universities, 2021, 42(3): 731.
[3]	MA Zihui, WANG Mengyan, CAO Hongyu, TANG Qian, WANG Lihao, ZHENG Xuefang. Transient Absorption and Decay Kinetic Properties of Photo-excited Metal Coordinated Tetraphenylporphyrin [J]. Chem. J. Chinese Universities, 2021, 42(3): 767.
[4]	ZHANG Aiqin, WANG Man, SHEN Gangyi, JIN Jun. Interactions Between Polybrominated Diphenyl Ethers and Human Serum Albumin Using SPR and Molecular Docking [J]. Chem. J. Chinese Universities, 2020, 41(9): 2054.
[5]	CAO Hongyu,MA Zihui,ZHANG Wenqiong,TANG Qian,LI Ruyu,ZHENG Xuefang. Structures and Electronic Absorption Spectra of N/Neo-Confused, Doubly N-Confused and Neo-Confused N-Confused Porphyrin Isomers ^† [J]. Chem. J. Chinese Universities, 2020, 41(2): 341.
[6]	WANG Qian, YANG Zheng, FANG Zheng. Biological Tissue Recognition Based on X-Ray Absorption Spectral Detection^† [J]. Chem. J. Chinese Universities, 2018, 39(7): 1434.
[7]	CUI Shengfeng, WAN Jingwei, ZHOU Chenghe. Fluorescence Spectroscopic Analysis on the Synergistic Mechanism of Diazepam and Ethanol^† [J]. Chem. J. Chinese Universities, 2018, 39(6): 1178.
[8]	HAO Yuanyuan, WU Qi, LI Ji, GE Chao, MA Chaoying, QIAN Yong, SU Zhi, LIU Hongke. Novel Os^Ⅱ-arene Complexes Based on Bipyridyl Derivative Ligands: Synthesis, Crystal Structure, Anticancer Activity and Interaction with DNA/BSA^† [J]. Chem. J. Chinese Universities, 2018, 39(4): 614.
[9]	WANG Yan, CHEN Ping, WANG Yunfei, LIU Guiying, YANG Xi, SU Ying, LI Junyang, LIU Weiwei, LIN Lie. Spectral Characterization of the Interaction Between Methamphetamine and Serum Albumin^† [J]. Chem. J. Chinese Universities, 2018, 39(11): 2507.
[10]	AN Pengjiao, YU Nannan, SUN Ruisheng, SUI Xiaofang, SONG Yuguang. Characterization of the Interaction Between Esterified TAM Radical and Bovine Serum Albumin [J]. Chem. J. Chinese Universities, 2017, 38(8): 1354.
[11]	XU Guoqing, HAO Changchun, HE Jianzhen, ZHANG Lei, SUN Runguang. Effect of Bovine Serum Albumin on the Structure of DSPE Monolayer^† [J]. Chem. J. Chinese Universities, 2017, 38(12): 2238.
[12]	TANG Qian, SU Jinhong, CAO Hongyu, WANG Lihao, SHI Fei, WANG Ailing, GONG Tingting, JIN Xiaojun, ZHENG Xuefang. Interaction of Pyrimidine Derivatives with Human Serum Albumin^† [J]. Chem. J. Chinese Universities, 2017, 38(11): 1982.
[13]	ZHANG Jing, CHEN Linfeng, ZHU Yaxian, ZHANG Yong. Effects of Hydroxypropyl-β-cyclodextrin(HPCD) on the Interaction of 1-Hydroxypyrene with Bovine Serum Albumin^† [J]. Chem. J. Chinese Universities, 2017, 38(1): 28.
[14]	SUN Linlin, ZHANG Ting, YAN Likai, SU Zhongmin. Theoretical Studies on Performance of Transiton Metal-substituted Keggin-type Phosphotungstate Dyes for Dye-sensitized Solar Cells^† [J]. Chem. J. Chinese Universities, 2016, 37(3): 529.
[15]	ZHANG Jing, CHEN Weixiao, ZHANG Wei, DUAN Ying, ZHU Yuxiu, ZHU Yaxian, ZHANG Yong. Interaction of 1-Hydroxypyrene with BSA Using Fluorescence Anisotropy and Synchronous Fluorescence Analysis Methods^† [J]. Chem. J. Chinese Universities, 2015, 36(8): 1511.

Interaction of Proteins with Fe(Ⅲ)-Chrome Azurol S Complex

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments