Identification and Mass Spectrometric Characterization of Isomeric Isoflavone Aglycones by ESI-IT-TOF Mass Spectrometry

Chem. J. Chinese Universities

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Identification and Mass Spectrometric Characterization of Isomeric Isoflavone Aglycones by ESI-IT-TOF Mass Spectrometry

LI Hui¹, WAN Le-Ren², WANG Hong¹, Hashi Yuki²*, CHEN Shi-Zhong¹*

1. School of Pharmaceutical Sciences, Peking University, Beijing 100083, China;
2. Shimadzu International Trading(Shanghai) Co. Limited, Beijing Office, Beijing 100020, China

Received:2007-03-27 Revised:1900-01-01 Online:2007-12-10 Published:2007-12-10
Contact: CHEN Shi-Zhong

Abstract

Abstract: A mass spectrometric method based on the combined with electrospray ionization/ion trap/time-of-flight(ESI-IT-TOF) mass spectrometer was used for systematic research of 7-methoxy-4'-hydroxy isoflavone and formononetin. MS⁵ spectra both in positive and negative ion modes with a high resolution were obtained. Different product ions were observed in positive ion mode, while almost the same product ions were observed in negative ion mode. With the high selectivity of parent ions and precise formulae of each fragment, fragmentation mechanisms were proposed with a high reliability. The established method could be used for the sensitive and rapid identification of isoflavonoids.

Key words: 7-Methoxy-4’-hydroxy isoflavone, Formononetin, Isomeric compound, Mass spectrometric characteristics of isoflavones, Ion trap/time-of-flight mass spectrometer

CLC Number:

O657.63

TrendMD:

LI Hui¹, WAN Le-Ren², WANG Hong¹, Hashi Yuki²*, CHEN Shi-Zhong¹*. Identification and Mass Spectrometric Characterization of Isomeric Isoflavone Aglycones by ESI-IT-TOF Mass Spectrometry[J]. Chem. J. Chinese Universities, doi: .

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Identification and Mass Spectrometric Characterization of Isomeric Isoflavone Aglycones by ESI-IT-TOF Mass Spectrometry

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