Abstract: Poly( N-hydroxyethyl acrylamide) resin was prepared by reacting ethanolamine with crosslinked poly(methyl acrylate). α Hydroxyl chloroethyl groups were introduced into the resin by treating the resin with epichlorohydrin with the acid catalysis. Three adsorbents with D-alanine, Lananine or glycine, respectively, were made by reacting the α hydroxyl chloroethyl group containing resin with the corresponding amino acids. The results of adsorbing N-demethylvan-comycin of the adsorbents indicated that the adsorbent with D-alanine had the highest adsorption capacity, the second was the adsorbent with glycine, while the adsorbent with Lalanine didn't adsorb N-demethylvan-comycin. The optimal pHfor the adsorption for adsorbent with D-alanine was ～5.8 and the adsorption capacity decreased with increasing salt(NaCl) concenttration. The adsorbed N-demethylvan-comycin was completely desorbed with 0.4 mmol/L Na 2CO₃/CH₃CN(molar ratio 7∶3, pH=9.5).

Key words: Affinity, Adsorption, Vancomycin, N Demethylvancomycin, Polymer