Separation, Purification and Structure Characterization of Polysaccharide from Roots of Cudrania tricuspidata(Carr.) Bur.

Chem. J. Chinese Universities

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Separation, Purification and Structure Characterization of Polysaccharide from Roots of Cudrania tricuspidata(Carr.) Bur.

SHI Lei^1,2, CHEN Kao-Shan¹, DONG Qun², FANG Ji-Nian², DING Kan²*

1. School of Life Science, Shandong University, Jinan 250100, China;
2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China

Received:2006-07-12 Revised:1900-01-01 Online:2007-06-10 Published:2007-06-10
Contact: DING Kan

Abstract

Abstract: A water-soluble polysaccharide(CPS-0) was obtained from the root of Cudrania tricuspidata(Carr.) Bur., by hot water extraction(70 ℃), deproteination with enzymolysis and Sevag method, precipitation with ethanol, and fractionation through DEAE-Sephadex A-50 chromatography. The purity of CPS-0 was determined via HPLC and the structure was characterized with monosaccharide composition analysis, methylation analysis, GC, GC-MS, NMR spectra(¹H NMR, ¹³C NMR, HMQC), UV, IR, and elemental analysis. The CPS-0 was found to contain glucose residue solely, with an average repeating unit of decasaccharide, ha-ving a backbone consisting of 1,4-linked α-D-glucopyranosyl residues, to which the side chain consisting of terminal and 1,4-linked α-D-glucopyranosyl residues was attached at position 6 of the branching residues.

Key words: Root of Cudrania tricuspidata(Carr.) Bur., Polysaccharide, Separation and purification, Structure characterization

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SHI Lei^1,2, CHEN Kao-Shan¹, DONG Qun², FANG Ji-Nian², DING Kan²*. Separation, Purification and Structure Characterization of Polysaccharide from Roots of Cudrania tricuspidata(Carr.) Bur.[J]. Chem. J. Chinese Universities, doi: .

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Separation, Purification and Structure Characterization of Polysaccharide from Roots of Cudrania tricuspidata(Carr.) Bur.

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