Studies and Application of Polarographic Catalytic Wave of Lysozyme in the Presence of KIO3

Abstract

Abstract: Apolarographic catalytic wave of lysozyme(LE) in the presence of KIO₃is described. In 0.1 mol/L HAc-NaAc(pH4.7±0.1) buffer solution, LEyields a reversible wave with peak potential -0.51 V (vs. Ag/AgCl) due to the reduction of a disulfide bond between cys6-cys127. When KIO₃is present in the acetate solution, LEproduced a polarographic catalytic wave at original potential. The mechanism of the catalytic wave was considered that the disulfide bond was regenerated through oxidation reaction of reduction product -SHgroup by KIO₃and its derived species with intermediate valence state(including free radical IO^-·, IO_{2^-·^{). This is a new class of polarographic catalytic wave for protein. In₀₁mol/L HAc-NaAc(pH4.7±0.1)-1×10^-3mol/L KIO₃supporting electrolyte, the sensitivity of the catalytic wave is two orders of magnitude higher than that of the corresponding reduction wave. The peak current of the catalytic wave was linearly proportional to LEconcentration in the range 2×10^-7-1×10^-6mol/L. 100-fold cystine and cystein do not interfere the determination of 1×10^-6mol/L LE.}}

Key words: Protein, Lysozyme, KIO₃, Polarographic catalytic wave

CLC Number:

O657.1

TrendMD:

GUO Wei, YANG Yia-Ni, SONG Jun-Feng. Studies and Application of Polarographic Catalytic Wave of Lysozyme in the Presence of KIO₃[J]. Chem. J. Chinese Universities, 2000, 21(2): 198.

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Studies and Application of Polarographic Catalytic Wave of Lysozyme in the Presence of KIO₃

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