ss-DNA Immobilization and Hybridization on Nano-Gold/ 2-Aminoethane Thoil/Glassy Carbon Electrode

Abstract

Abstract: The modification of glassy carbon electrode(GCE) with nano-gold(NG) by chemical adsorption of 2-aminoethane thoil(AET) was achieved for the first time. On this electrode 15-mer ss-DNA was immobilized, then ss-DNA/NG/AET/GCE was formed. By using Co(bpy)₃³⁺, hybridization events of the immobilized ss-DNA was well detected. It was found that the NG could possibly cause a part of the ss-DNA to denature and bind Co(bpy)₃³⁺. However, soaking in pH 7.0 phosphate buffer solution, denaturization can be avoided and recognition ability of hybridization was enhanced. Cyclic voltammetric behavior of Co(bpy)₃^3+/2+ bound to the ds-DNA/NG/AET/GCE was studied. It was found that the peak current(i_p) showed a linear dependence on the potential scan rate up to 80 mV/s. It was demonstrated that GCE modified with NG by this method had higher stability and reliability.

Key words: DNA, Nano-gold, Modified electrode, Hybridization, Cyclic voltammetry

CLC Number:

O626.11

TrendMD:

MIAO Qian, JIN Bao-Kang, LIN Xiang-Qin . ss-DNA Immobilization and Hybridization on Nano-Gold/ 2-Aminoethane Thoil/Glassy Carbon Electrode[J]. Chem. J. Chinese Universities, 2000, 21(1): 27.

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ss-DNA Immobilization and Hybridization on Nano-Gold/ 2-Aminoethane Thoil/Glassy Carbon Electrode

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