Preparation of Zwitterionic Ion Exchange Packings Based on Monodisperse Hydrophilic Macroporous Resins and Their Application to Separation of Proteins

Chem. J. Chinese Universities

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Preparation of Zwitterionic Ion Exchange Packings Based on Monodisperse Hydrophilic Macroporous Resins and Their Application to Separation of Proteins

GONG Bo-Lin^1,2*, REN Li¹, YAN Chao², HU Wen-Zhi³

1. Key Laboratory of Energy & Chemical Engineering, Ningxia University, Yinchuan 750021, China;
2. Unimicro(Shanghai) Technologies Co., Ltd., Shanghai 201203, China;
3. Laboratory of Bio-Functional Chemistry, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan

Received:2006-08-10 Revised:1900-01-01 Online:2007-05-10 Published:2007-05-10
Contact: GONG Bo-Lin

Abstract

Abstract: Monodisperse, macroporous hydrophilic poly(glycidylmethacrylate-co-ethylene dimethacrylate) particles with a diameter of 5.0 μm were prepared via a one-step swelling method combined with dispersion polymerization and technique of polymeric solution porogens, then they were characterized with various measurements. The resins were modified to be strong/strong ziwitterioic stationary phase for high performance liquid chromatography(HPLC) in the following steps. First, a tertiary amine was introduced on the particles by dimethylamination, followed by quaternizing sulfoalkylation with 1,3-propane sultone. The zwitterionic ion exchange stationary phase was evaluated in light of the separability and hydrophilicity on the separation and retention of standard proteins in detail. Three acid proteins and two basic ones were quickly separated in 4.0 min with 3 mL/min linear gradient elution by using the synthesized zwitterionic ion exchange stationary phase.

Key words: Monodisperse macroporous hydrophilic polymeric beads, Zwitterionic ion exchange packings, Proteins separation

CLC Number:

O658

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GONG Bo-Lin^1,2*, REN Li¹, YAN Chao², HU Wen-Zhi³. Preparation of Zwitterionic Ion Exchange Packings Based on Monodisperse Hydrophilic Macroporous Resins and Their Application to Separation of Proteins[J]. Chem. J. Chinese Universities, doi: .

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Preparation of Zwitterionic Ion Exchange Packings Based on Monodisperse Hydrophilic Macroporous Resins and Their Application to Separation of Proteins

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