Multilayer Assembly Based on Concanavalin-Glycoprotein Specific Interaction and Its Application to the Preparation of Glucose Biosensors

Abstract

Abstract: The multilayers were constructed by the strong lection glycoprotein specific interaction and were used in the preparation of glucose biosensors. The results showed that the glucose oxidase with glucose residues and hydrogen peroxidase without glucose residues could be assembled into multilayers. The thickness of multiplayer films increased linearly with the increase of the layers. The morphology was investigated, and the relationship between film structure and function was discussed. The study on electrochemistry of gold electrode of lectin enzyme multiplayer shows that the enzyme in multilayers remains the catalytic activity.

Key words: Concanavalin, Glycoprotein, Layer-by-layer assembly, Biosensor

CLC Number:

O631

TrendMD:

SHI Li-Xin, LU Ying-Xi, ZHANG Jing, LIU Jun-Qiu, SHEN Jia-Cong . Multilayer Assembly Based on Concanavalin-Glycoprotein Specific Interaction and Its Application to the Preparation of Glucose Biosensors[J]. Chem. J. Chinese Universities, 2004, 25(3): 575.

[1]	WANG Junyang, LIU Zheng, ZHANG Qian, SUN Chunyan, LI Hongxia. Application of DNA Silver Nanoclusters in the Fluorescence Biosensors based on Functional Nucleic Acids [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220010.
[2]	XU Mengyi, HUANG Xuewen, LI Xiaojie, WEI Wei, LIU Xiaoya. Fabrication of Biosensor Based on “Beads-on-a-String” Shaped Composite Nano-assembly Modified Screen Printed Electrode [J]. Chem. J. Chinese Universities, 2021, 42(6): 1768.
[3]	WU Yangyi, CHEN Jianping, Ai Yijing, WANG Qingxiang, GAO Fei, GAO Feng. Synthesis of 2-（2-Hydroxy-3-methoxyphenyl）-C₆₀ and Its Application for Sensing of Cauliflower Mosaic Virus 35S Promotor [J]. Chem. J. Chinese Universities, 2021, 42(6): 1754.
[4]	WANG Bodong, PAN Meichen, ZHUO Ying. Construction of Electrochemiluminescence Sensing Interface Based on Silver Nanoclusters-Silica Nanoparticles and Biomolecular Recognition [J]. Chem. J. Chinese Universities, 2021, 42(11): 3519.
[5]	ZHANG Jiayi, DING Zhenyao, WANG Dandan, CHEN Liping, FENG Xinjian. Fabrication of Triphase Enzyme Electrode Based on Porous Gold Substrate for High-performance Electrochemical Biosensor [J]. Chem. J. Chinese Universities, 2021, 42(10): 3167.
[6]	YUAN Zhongwen, HE Lizhen, CHEN Tianfeng. Biomedical Applications of Single-atom Catalysts [J]. Chem. J. Chinese Universities, 2020, 41(12): 2690.
[7]	ZHANG Yimeng, ZHANG Huixin, LIU Yang. Recent Advances of Exosomes Bioanalysis and Their Clinic Applications [J]. Chem. J. Chinese Universities, 2020, 41(11): 2306.
[8]	ZHENG Shan, LIU Yang, CHEN Piaopiao, XING Yichen, HUANG Chaobiao. Novel Glutathione Photoelectrochemical Sensor Based on PbS QDs/TiO₂ NPs ^† [J]. Chem. J. Chinese Universities, 2019, 40(9): 1866.
[9]	ZHOU Yuting, TANG Yujiao, SHAO Shuang, DAI Shiyan, CHENG Guifang, HE Pingang, FANG Yuzhi. Simultaneous Detection of Mercury, Lead and Strontium Ions Based on Conformational Conversion Sensor ^† [J]. Chem. J. Chinese Universities, 2019, 40(8): 1621.
[10]	YANG Yanyan,TAN Jianing,FENG Suyang,QI Yue,WANG Yang,DING Yuansheng,QU Xiaoshu,LIAN Lili,YU Xiaoyang. Preparation and Properties of Vanadium Tungstate-CdS Nanoparticles Electrochromic Composite Films^† [J]. Chem. J. Chinese Universities, 2019, 40(4): 659.
[11]	JIA Hongliang,ZHAO Jianwei,QIN Lirong,ZHAO Min. Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets^† [J]. Chem. J. Chinese Universities, 2019, 40(2): 240.
[12]	WANG Chunyan,JIANG Xiaoqing,ZHOU Bo. An Electrochemical Biosensor Based on Cu-TPA for Determination of Aflatoxin B1 ^† [J]. Chem. J. Chinese Universities, 2019, 40(11): 2301.
[13]	LI Cheng,WANG Chengjian,JIN Wanjun,HAN Jianli,YANG Meifang,GAO Xi,HUANG Linjuan,WANG Zhongfu. Mass Spectrometric Analysis of N-Glycans of Glycoprotein Separated by SDS-PAGE Gel from Ginkgo Seed^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 69.
[14]	YANG Hui, LI Tong, TONG Weijun, GAO Changyou. Fabrication of Microcapsules with Special Shapes by Layer-by-layer Assembly on CaCO₃ Microparticles^† [J]. Chem. J. Chinese Universities, 2018, 39(1): 172.
[15]	HE Wenli, CHEN Meng, QIAN Dongjin. Pd(Ⅱ)-Directed Layer-by-layer Assembly of Multiporphyrin Arrays on Silica Nanoparticle Surfaces for Photocurrent Generation and Photochromism of Viologens^† [J]. Chem. J. Chinese Universities, 2017, 38(9): 1654.

Multilayer Assembly Based on Concanavalin-Glycoprotein Specific Interaction and Its Application to the Preparation of Glucose Biosensors

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments