Interaction Between Domoic Acid and Double-Stranded Oligodeoxy-nucleotide Studied by Using Microchip-based Non-gel Sieving Electrophoresis

Chem. J. Chinese Universities ›› 2005, Vol. 26 ›› Issue (7): 1252.

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Interaction Between Domoic Acid and Double-Stranded Oligodeoxy-nucleotide Studied by Using Microchip-based Non-gel Sieving Electrophoresis

ZHOU Xiao-Mian^1,2, LI Da-Zhi^1,3, SHEN Zheng¹, LIU Wei³, LI Gui-Ru⁴, LIN Bing-Cheng¹

1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Depart_ment of Clinical Laboratory, Guangzhou First Municipal People's Hospital, Affiliated of Guangzhou Medical College, Guangzhou 510500, China;
3. Dalian Customs Laboratory, Dalian 116001, China;
4. The Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China

Received:2004-09-04 Online:2005-07-10 Published:2005-07-10

Abstract

Abstract: The interaction between domoic acid(DA) and 18-mer double-stranded DNA(dsDNA) was investigated by using microchip-based non-gel sieving electrophoresis. For the 18-mer dsDNA with a sequence of 3'-GCATTGGTTGACGTTGCA-5', the amount of free dsDNA decreases with the increase of the added DA and also with the increase of reactive time. Meanwhile, the newly-produced two peaks were observed. The experimental results show that there is a strong interaction between DA and the 18-mer dsDNA. DA makes the degradation of the 18-mer dsDNA. The non-gel sieving electrophoresis on microchip is a rapid, convenient, highly sensitive method for studying the interaction between DNA and small molecules.

Key words: Microchip non-sieving electrophoresis, Interaction, Domoic acid, 18-mer dsDNA

CLC Number:

O657

TrendMD:

ZHOU Xiao-Mian, LI Da-Zhi, SHEN Zheng, LIU Wei, LI Gui-Ru, LIN Bing-Cheng. Interaction Between Domoic Acid and Double-Stranded Oligodeoxy-nucleotide Studied by Using Microchip-based Non-gel Sieving Electrophoresis[J]. Chem. J. Chinese Universities, 2005, 26(7): 1252.

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Interaction Between Domoic Acid and Double-Stranded Oligodeoxy-nucleotide Studied by Using Microchip-based Non-gel Sieving Electrophoresis

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