Chem. J. Chinese Universities

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Preparation of Bovine Serum Albumin Capillary Monolith for the Separation of Histidine Enatiomers

ZHU Tao-Yu, WU Pin-Duan, ZUO Na-Na, WU Jing-Hong, MA Zhi-Ling   

  1. School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China
  • Received:2006-10-12 Revised:1900-01-01 Online:2007-03-10 Published:2007-03-10
  • Contact: MA Zhi-Ling

Abstract: A series of monolithic capillary columns were prepared by copolymerization of GMA and EDMA in the presence of a porogenic solvent containing cyclohexanol and n-dodecanol. The influences of the contents of the porogenic solvent and GMA on the monolith morphology and osmosis were investigated. The capillary monolith matrixes were prepared under the optimized conditions, and BSA capillary monoliths were gained by epoxy method using BSA as the model ligand. The BSA capillary monolith coupled with capillary electrochromatography was developed for the separation of D,L-histidine, and showed a good result.

Key words: Capillary monolith, Bovine serum albumin, Histidine enantiomer

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