Study on Enzyme Mimics of Molecularly Imprinted Microgels

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (12): 2488.

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Study on Enzyme Mimics of Molecularly Imprinted Microgels

WANG Hong-Fei^1,2, TANG Chun-Yan¹, YANG Hao¹, ZHANG Li-Ming^1,2*

1. Key Laboratory of Designed Synthesis and Application of Polymer Materials, Sun Yat\|Sen University, Guangzhou 510275, China
2. Key Laboratory for Polymeric Composite and Functional Materials, Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat\|Sen University, Guangzhou 510275, China

Received:2010-02-23 Revised:2010-05-17 Online:2010-12-10 Published:2010-12-06
Contact: Li-Ming Zhang E-mail:ceszhlm@mail.sysu.edu.cn
About author:张黎明, 男, 博士, 教授, 博士生导师, 主要从事生物功能材料的研究.
Supported by:
国家自然科学基金(批准号: 20874116, 20676155)和教育部高等学校博士点基金(批准号: 20090171110023)资助.

Abstract

Abstract: Molecularly imprinted microgels (MIGs) exhibiting selective esterase activity were prepared by a reverse emulsion method using maleic anhydride esterified dextran-aminopyridine conjugate (Dex-MA-AP) as the functional macromonomer, p-nitrophenyl phosphate (NPP) as the stable transition state analogue (TSA) and Co2+ as the coordination center. The interaction between Dex-MA-AP and NPP was investigated by UV adsorption spectra. The morphology and size of MIGs were observed by scanning electron micrographs (SEM). It was found that the catalytic activity of MIGs was greatly influenced by the amounts of the template and cross-linking agent. The hydrolysis kinetics of p-nitrophenyl acetate (NPA) in the presence of MIGs could be described by the Michaelis-Menten equation. The maximium velocity (Vm) and Michaelis-Menten constant (Km) were found to be 25.1 mmol/h and 0.030 mmol/L, respectively. In addition, the MIGs was found to show high catalytic selectivity to NPA.

Key words: Enzyme mimics, Molecularly imprinting, Microgels, Catalytic activity, Catalytic selectivity

TrendMD:

WANG Hong-Fei, TANG Chun-Yan, YANG Hao, ZHANG Li-Ming. Study on Enzyme Mimics of Molecularly Imprinted Microgels[J]. Chem. J. Chinese Universities, 2010, 31(12): 2488.

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Study on Enzyme Mimics of Molecularly Imprinted Microgels

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