Preparation and Structure Characterization of an Alkali-soluble Polysaccharide from Grifola frondosa Mycelium

Chem. J. Chinese Universities

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Preparation and Structure Characterization of an Alkali-soluble Polysaccharide from Grifola frondosa Mycelium

YU Guang-Li, WANG Ying, ZHAO Xia, GAO Hao-Dong, GUAN Hua-Shi

Marine Drug and Food Institute, Lab of Glycochemistry & Glycotechnology, Ocean University of China, Qingdao 266003, China

Received:2005-12-15 Revised:1900-01-01 Online:2007-01-10 Published:2007-01-10
Contact: YU Guang-Li

Abstract

Abstract: An alkali-soluble polysaccharide(GFM2A)was extracted by cold sodium hydroxide and further purified by column chromatography from fermented Grifola frondosa mycelium. The structural characterization of GFM2A was confirmed by NaIO₄ oxidation, Smith degradation, acetolysis, GCMS, IR and NMR techniques. The results showed that GFMZA contain glucose(Glc),mannose(Man)and xylose(Xyl), and their molar ratio was 9∶2∶1. There are six α-1,3-D-Glc and two α-1,3-D-Man in the backbone, and there are two branches in the main chain, and both of them linked to α-1,3-D-Glc residue, one branch contains three β-1,4-D-Glc, another branch contains one α-1,4-D-Xyl.

Key words: Grifola frondosa mycelium, Polysaccharide, Preparation, Structure characterization

CLC Number:

O62
TQ464.1

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YU Guang-Li, WANG Ying, ZHAO Xia, GAO Hao-Dong, GUAN Hua-Shi. Preparation and Structure Characterization of an Alkali-soluble Polysaccharide from Grifola frondosa Mycelium[J]. Chem. J. Chinese Universities, doi: .

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Preparation and Structure Characterization of an Alkali-soluble Polysaccharide from Grifola frondosa Mycelium

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