Structural Characterization of a Homogeneous Polysaccharide Isolated from the Flower of Carthamus Tinctorius L. and Its Inhibitory Activity on HepG2 Proliferation

doi:10.7503/cjcu20220600

Abstract

Abstract:

To isolate， purify and characterize the structure of polysaccharide from flowers of Carthamus tinctorius L.， and to investigate the inhibitory effect of its homogeneous polysaccharide on the proliferation of HepG2 hepatoma cells. The polysaccharides in flowers of Carthamus tinctoriusL. were extracted by water extraction and alcohol precipitation. The crude polysaccharides were separated and purified by anion exchange column and molecular sieve gel column. The homogeneity and molecular weight distribution were determined by high performance gel permeation chromatography（HPGPC）. Its structure was elucidated by monosaccharide composition， methylation， and nuclear magnetic resonance（NMR） methods. The inhibitory effect of flowers of Carthamus tinctoriusL. polysaccharide on tumor cells was studied by in vitro cell experiments. A homogeneous polysaccharide（CTPS-1A） was obtained. The molecular weight of CTPS-1A was 53800. The monosaccharide composition of CTPS-1A was analyzed by gas chromatography-mass spectrometer（GC-MS） and the monosaccharide composition was n（arabinose）∶n（galactose）=59.2∶40.8. By methylation analysis， the glycosidic linkages were T-Galp， 1，3-Galp， 1，6-Galp， 1，3，6-Galp， T-Araf， 1，5-Araf， and 1，3，5-Araf. The results showed that CTPS-1A was an arabinogalactan（AG）， its main chain was composed of -β-Galp-（6→1）-β-Galp-（6→， and T-β-Galp， 1，3-β-Galp， T-α-Araf， 1，5-α-Araf， 1，3，5-α-Araf were connected to the 3 position of 1，3，6-Galp. The results of in vitro culture of HepG2 cells showed that CTPS-1A inhibited the growth of HepG2 by enhancing nuclear contraction and apoptosis.

Key words: Carthamus tinctoriusL, Polysaccharide, Structural characterization, Arabinogalactan, HepG2 hepatoma cell

CLC Number:

O629.12

TrendMD:

XU Yongbin, FENG Shuaixia, CHEN Jie, GONG Huan, SHI Songshan, WANG Huijun, WANG Shunchun. Structural Characterization of a Homogeneous Polysaccharide Isolated from the Flower of Carthamus Tinctorius L. and Its Inhibitory Activity on HepG2 Proliferation[J]. Chem. J. Chinese Universities, 2023, 44(4): 20220600.

Figures/Tables 10

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Methylated suger	Linkage type	Molar fration（%）
2，3，5⁃Me₃⁃Araf	T⁃Araf	24.8
2，3⁃Me₂⁃Araf	1，5⁃Araf	29.8
3⁃Me₁⁃Araf	1，3，5⁃Araf	4.7
2，3， 4，6⁃Me₄⁃Galp	T⁃Galp	4.2
2，4，6⁃Me₃⁃Galp	1，3⁃Galp	3.1
2，3，4⁃Me₃⁃Galp	1，6⁃Galp	14.3
2，4⁃Me₂⁃Galp	1，3，6⁃Galp	19.1

Methylated suger	Linkage type	Molar fration（%）
2，3，5⁃Me₃⁃Araf	T⁃Araf	24.8
2，3⁃Me₂⁃Araf	1，5⁃Araf	29.8
3⁃Me₁⁃Araf	1，3，5⁃Araf	4.7
2，3， 4，6⁃Me₄⁃Galp	T⁃Galp	4.2
2，4，6⁃Me₃⁃Galp	1，3⁃Galp	3.1
2，3，4⁃Me₃⁃Galp	1，6⁃Galp	14.3
2，4⁃Me₂⁃Galp	1，3，6⁃Galp	19.1

δ
Residue	C⁃1/H⁃1	C⁃2/H⁃2	C⁃3/H⁃3	C⁃4/H⁃4	C⁃5/H⁃5	C⁃6/H⁃6
T⁃α⁃Araf	110.3/5.24	82.5/4.22	77.9/4.01	85.0/4.14	62.2/3.72， 3.83	—/—
1，5⁃α⁃Araf	108.6/5.09	81.9/4.13	77.9/3.96	85.0/4.08	67.9/3.82， 3.90	—/—
1，3，5⁃α⁃Araf	108.1/5.15	82.5/4.14	85.1/4.09	83.2/4.3	68.0/3.84， 3.93	—/—
T⁃β⁃Galp	105.4/4.62	74.0/3.64	74.8/3.88	70.6/4.02	76.7/3.73	62.4/3.82， 3.84
1，3⁃β⁃Galp	104.9/4.69	73.1/3.78	81.2/3.74	70.1/3.89	75.9/3.81	62.3/3.89， 3.83
1，6⁃β⁃Galp	104.3/4.46	73.0/3.55	75.6/3.70	71.0/3.64	74.6/3.70	70.1/3.74， 3.82
1，3，6⁃β⁃Galp	104.4/4.52	73.8/3.66	83.4/3.91	69.9/4.13	74.9/3.94	69.9/3.73， 3.84

δ
Residue	C⁃1/H⁃1	C⁃2/H⁃2	C⁃3/H⁃3	C⁃4/H⁃4	C⁃5/H⁃5	C⁃6/H⁃6
T⁃α⁃Araf	110.3/5.24	82.5/4.22	77.9/4.01	85.0/4.14	62.2/3.72， 3.83	—/—
1，5⁃α⁃Araf	108.6/5.09	81.9/4.13	77.9/3.96	85.0/4.08	67.9/3.82， 3.90	—/—
1，3，5⁃α⁃Araf	108.1/5.15	82.5/4.14	85.1/4.09	83.2/4.3	68.0/3.84， 3.93	—/—
T⁃β⁃Galp	105.4/4.62	74.0/3.64	74.8/3.88	70.6/4.02	76.7/3.73	62.4/3.82， 3.84
1，3⁃β⁃Galp	104.9/4.69	73.1/3.78	81.2/3.74	70.1/3.89	75.9/3.81	62.3/3.89， 3.83
1，6⁃β⁃Galp	104.3/4.46	73.0/3.55	75.6/3.70	71.0/3.64	74.6/3.70	70.1/3.74， 3.82
1，3，6⁃β⁃Galp	104.4/4.52	73.8/3.66	83.4/3.91	69.9/4.13	74.9/3.94	69.9/3.73， 3.84

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