Fabrication of Biosensor Based on “Beads-on-a-String” Shaped Composite Nano-assembly Modified Screen Printed Electrode

doi:10.7503/cjcu20200700

Abstract

Abstract:

An amphiphilic random copolymer poly（AAPBA-co-FMA-co-HEMA）（PAFH） was synthesized with 3-acrylamidophenylboronic acid（AAPBA）， furfuryl methacrylate（FMA） and 2-hydroxyethyl methacrylate（HEMA） as monomers. Thereafter， the functional polymer covalently grafted carbon nanotubes（PAFH-CNTs） was fabricated via Diels-Alder reaction between PAFH and carbon nanotubes（CNTs）. They were co-assembled to form composite nano-assemblies（PAFH-CNTs NCs）. The morphology and stability of PAFH-CNTs NCs were measured by scanning electron microscope（SEM） and ultraviolet spectrophotometer. The results showed that PAFH-CNTs NCs has a zero dimensional/one dimensional（0D/1D） “beads-on-a-string” structure with long-term stability. Finally， PAFH-CNTs NCs were modified on the surface of screen-printed carbon electrode（SPCE）， and the horseradish peroxidase（HRP） was introduced to construct a biosensor to detect hydrogen peroxide（H₂O₂）. SEM， transmission electron microscopy（TEM） and electrochemical workstation were used to characterize the morphology and sensing performance of the biosensor. It was illustrated that PAFH-CNTs NCs could form a nanonetwork with high specific surface area on the SPCE surface. The constructed sensor has a good linear response to H₂O₂ within the range of 0.02—3.48 mmol/L， and the sensitivity and detection limit are 63.98 μA·（mmol/L）^-1·cm^-2 and 4.2 μmol/L， respectively. Furthermore， it also has excellent stability and selectivity， which has application value for testing actual samples.

Key words: Amphiphilic random copolymer, Carbon nanotubes, Composite nano-assembly, H₂O₂, Biosensor

CLC Number:

O657

TrendMD:

XU Mengyi, HUANG Xuewen, LI Xiaojie, WEI Wei, LIU Xiaoya. Fabrication of Biosensor Based on “Beads-on-a-String” Shaped Composite Nano-assembly Modified Screen Printed Electrode[J]. Chem. J. Chinese Universities, 2021, 42(6): 1768.

Figures/Tables 12

References 27

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Sample	c(Added)/(mmol?L^-1)	c(Found)/(mmol?L^-1)	Recovery rate(%)	RSD(%)
Milk 1	0.02	2.090×10^-2	104.5	2.43
Milk 2	0.1	9.784×10^-2	97.8	3.97
Milk 3	1	1.028×10⁰	102.8	3.33
Lake water 1	0.02	2.034×10^-2	101.7	1.52
Lake water 2	0.1	1.009×10^-1	100.9	2.70
Lake water 3	1	9.935×10^-1	99.4	1.82

Sample	c(Added)/(mmol?L^-1)	c(Found)/(mmol?L^-1)	Recovery rate(%)	RSD(%)
Milk 1	0.02	2.090×10^-2	104.5	2.43
Milk 2	0.1	9.784×10^-2	97.8	3.97
Milk 3	1	1.028×10⁰	102.8	3.33
Lake water 1	0.02	2.034×10^-2	101.7	1.52
Lake water 2	0.1	1.009×10^-1	100.9	2.70
Lake water 3	1	9.935×10^-1	99.4	1.82

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