Multifunctional DNA Nanoflowers for Autophagy Inhibition and Enhanced Antitumor Chemotherapy†

doi:10.7503/cjcu20200242

Abstract

Abstract:

Autophagy is an important process in eukaryotic cells, in which subcellular membranes undergo dynamic morphological changes, leading to the degradation of cellular proteins and cytoplasmic organelles. High levels of cellular autophagy could protect tumor cells from apoptosis and reduce the antitumor effect of chemotherapy drugs. In this work, we designed a multifunctional DNA nanoflower, which encode DNA aptamer and DNAzyme for targeted drug delivery and gene silencing to inhibit autophagy in tumor cells. Using RT-PCR, we verified that the designed DNA nanoflower can effectively cleave the mRNA of autophagy-related gene(ATG5) in tumor cells. The cytotoxicity and cell apoptosis assay also showed that the synthesized multifunctional DNA nanoflower could enhance antitumor chemotherapy.

Key words: DNA nanoflowers, Drug delivery, Autophagy, DNAzyme, Antitumor chemotherapy

CLC Number:

O651
O636.9

TrendMD:

ZHANG Kaixiang, LIU Junjie, SONG Qiaoli, WANG Danyu, SHI Jinjin, ZHANG Haiyue, LI Jinghong. Multifunctional DNA Nanoflowers for Autophagy Inhibition and Enhanced Antitumor Chemotherapy^†[J]. Chem. J. Chinese Universities, 2020, 41(7): 1461.

Figures/Tables 7

References 35

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Sample	Sequence(5'- 3')
ATG5 DNAzyme 1	CAATCCCAGGCTAGCTACAACGA CCAGAG
ATG5 DNAzyme 2	TGCAATCCCAGGCTAGCTACAACGACCAGAGTTG
ATG5 F	FAM-CCAGAGTTGCTTGTGATCTT
ATG5 Q	CTGTCATTTTGCAATCCCA-BHQ
ATG5 substrate	AAGATCACAAGCAACTCTGG/rA/rU/GGGATTGCAAAATGACAG
Circle	CACAACCACCACCACCAACAACTCTGGTCGTTGTAGCTAGCCTGGGATTGCAAACCACCAC
Primer	GTGGTGGTTGTGGTGGTGGTGGTT
Random circle	CACGCTCACCTCCACCAACATAAATGCAAATAGAAACTAGGCTTTGATAATAAAGGTCCAGGAC
Random primer	GAGGTGAGCGTGGTCCTGGACCTT

Sample	Sequence(5'- 3')
ATG5 DNAzyme 1	CAATCCCAGGCTAGCTACAACGA CCAGAG
ATG5 DNAzyme 2	TGCAATCCCAGGCTAGCTACAACGACCAGAGTTG
ATG5 F	FAM-CCAGAGTTGCTTGTGATCTT
ATG5 Q	CTGTCATTTTGCAATCCCA-BHQ
ATG5 substrate	AAGATCACAAGCAACTCTGG/rA/rU/GGGATTGCAAAATGACAG
Circle	CACAACCACCACCACCAACAACTCTGGTCGTTGTAGCTAGCCTGGGATTGCAAACCACCAC
Primer	GTGGTGGTTGTGGTGGTGGTGGTT
Random circle	CACGCTCACCTCCACCAACATAAATGCAAATAGAAACTAGGCTTTGATAATAAAGGTCCAGGAC
Random primer	GAGGTGAGCGTGGTCCTGGACCTT