Glucose Oxidase Immobilization with Amino Dendritic Mesoporous Silica Nanoparticles and Its Application in Glucose Detection

doi:10.7503/cjcu20200146

Abstract

Abstract: Dendritic mesoporous silica nanoparticles(DMSNs) were synthesized with triethylamine as template and modified with 3-aminopropyl triethoxylsilane(APTES). Glucose oxidase(GOD) was immobilized by a Amino dendritic mesoporous silica nanoparticles(DMSNs-NH₂). The immobilized glucose oxidase(DMSNs-NH₂-GOD) was characterized by scanning electron microscopy, transmission electron microscopy, infrared spectroscopy, nitrogen adsorption apparatus and thermogravimetry. The activity and protein loading of immobilized glucose oxidase were determined. The results showed that the diameter of immobilized GOD was about 200 nm, the shape was uniform, and immobilized GOD was dispersed as spherical particles. Under the optimal fixation conditions, the protein loading reached 225 mg/g and the enzyme activity reached 215 U/mg. The minimum detection limit for glucose detection by immobilized GOD is 0.0014 mg/mL. Immobilized GOD was used to detect glucose in serum and beverage, which could be reused for more than 36 times, with the relative enzyme activity remaining 80%. The method is easy to operate and accurate. In the same time, it improves the pH stability, thermal stability, reusability of the enzyme and reduces the cost.

Key words: Dendritic mesoporous silica nanoparticles, Immobilized enzyme, Glucose oxidase, Glucose

CLC Number:

O613
R115

TrendMD:

WANG Huan, SUO Jinquan, WANG Chunyan, WANG Runwei. Glucose Oxidase Immobilization with Amino Dendritic Mesoporous Silica Nanoparticles and Its Application in Glucose Detection[J]. Chem. J. Chinese Universities, 2020, 41(8): 1731.

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