Controllable Preparation of Polydopamine Modified Gold Nanoflowers and Its Application in Photothermal Therapy †

doi:10.7503/cjcu20190488

Abstract

Abstract:

Gold nanoflowers(AuNFs) were prepared through template-free method. The size and morphology of AuNFs were modulated by reaction temperature and the amount of reducing agent ascorbic acid. Furthermore, polydopamine(PDA) modification was adopt to improve the near-infrared(NIR) absorbance and biocompatibility of AuNFs. The size, morphology and optical properties of AuNFs and PDA modified AuNFs were characterized by transmission electron microscopy(TEM), dynamic light scattering(DLS) & Zeta potential analyzer and UV-Vis spectroscopy. The modification of PDA was characterized by Fourier transform infrared(FTIR) spectroscopy. The X-ray diffraction(XRD) analysis indicated that the crystal structure of AuNFs had no changes after PDA modification. The cytotoxicity of samples in vitro was evaluated by MTT assay. The results demonstrated that the lower temperature(0 ℃) is the proper reaction temperature to obtain the multi-branched structures of AuNFs. Meanwhile, increasing the concentration of reducing agent ascorbic acid would obtain the smaller AuNFs. The size of AuNFs ranged from 60—100 nm, and the wavelength of UV-Vis absorption peak was 575—650 nm. After PDA modification, the significantly red-shifted(over 80 nm) of UV-Vis absorption was found, and the absorption range in the near infrared region was significantly improved. The thickness of PDA layer outside AuNFs was about 8—14 nm through PDA concentration changes. Under 808 nm laser irradiation, the solution with PDA-AuNFs were rapidly heated to 57 ℃. In addition, the cell viability in vitro demonstrated that the PDA-AuNFs had lower cytotoxicity. After photothermal treatment with PDA-AuNFs, the cell survival of HeLa cells was about 10%. Therefore, the PDA-AuNFs had potential applications as photothermal reagents for tumor therapy.

Key words: Gold nanoflower, Polydopamine, Morphology, Near-infrared absorbance, Photothermal therapy

CLC Number:

O611.3

TrendMD:

WU Fengren,LIU Yongjia,LU Xuemin,ZHU Bangshang. Controllable Preparation of Polydopamine Modified Gold Nanoflowers and Its Application in Photothermal Therapy ^†[J]. Chem. J. Chinese Universities, 2020, 41(3): 465.

Figures/Tables 8

Fig.1 TEM images(A)—(E) and UV-Vis spectra(F) of AuNFs synthesized at different temperatures Temperature/℃: (A) 0; (B) 10; (C) 20; (D) 30; (E) 40.

Fig.2 TEM images of AuNFs synthesized with different amounts of AA V(AA)/μL: (A) 20; (B) 40; (C) 60; (D) 80.

Fig.3 Hydrodynamic diameter(A), zeta potential(B) and UV-Vis spectra(C) of AuNFs synthesized with 20, 40, 60 and 80 μL AA

Fig.4 FTIR spectra of AuNFs prepared with 20 μL AA(AuNFs20), AuNFs modified with 5 mg/mL DA·HCl(PDA-Au5) and DA·HCl(A) and XRD patterns of standard Au(JCPDS No. 89-3697), AuNFs20 and PDA-Au5(B)

Fig.5 TEM images of AuNFs20(A), PDA-Au1(B), PDA-Au2(C) and PDA-Au5(D)

Fig.6 Hydrodynamic diameters(A), zeta potentials(B) and UV-Vis spectra(C) of AuNFs 20(a), PDA-Au1(b), PDA-Au2(c) and PDA-Au5(d)

Fig.7 Thermal images(A) and temperature-time profiles(B) of H2O(a), AuNFs20(b) and PDA-Au5(c) under irradiation of 808 nm laser(1 W/cm2) for 10 min

Fig.8 Cytotoxicity of HeLa cells cultivated with AuNFs and PDA-Au5 without(A) or with(B) a 10 min laser(1 W/cm2)

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