Chem. J. Chinese Universities ›› 2019, Vol. 40 ›› Issue (11): 2301.doi: 10.7503/cjcu20190233
• Analytical Chemistry • Previous Articles Next Articles
WANG Chunyan,JIANG Xiaoqing,ZHOU Bo()
Received:
2019-04-22
Online:
2019-11-10
Published:
2019-08-20
Contact:
ZHOU Bo
E-mail:zhoubo@njnu.edu.cn
Supported by:
CLC Number:
TrendMD:
WANG Chunyan,JIANG Xiaoqing,ZHOU Bo. An Electrochemical Biosensor Based on Cu-TPA for Determination of Aflatoxin B1 †[J]. Chem. J. Chinese Universities, 2019, 40(11): 2301.
Fig.1 XRD patterns of CuTPA×DMF(CCDC-687690)(a) and Cu-TPA(b)(A), chemical structure of Cu-TPA(B), IR spectra of HTPA(a), Cu-TPA(b) and Cu-TPA/AuNPs(C) and TEM image of Cu-TPA(D)
Fig.2 CV responses of the electrodes GE and Cu-TPA/GE in the scan range of -0.8—0.8 V(A) and DPV responses of the electrodes GE, Cu-TPA/GE and Cu-TPA/AuNPs/GE in Tris-HCl(pH=7.4) with a scan rate of 100 mV/s in the scan range of -0.1—0.5 V(B)
Fig.3 DPV responses of the CuTPA/AuNPs/S1/S3/S2/GE electrode to different concentrations of AFB1 in Tris-HCl(pH=7.4) with a scan rate of 100 mV/s and scan range of -0.1—0.5 V Concentration of AFB1/(ng·mL-1): a. 0; b. 0.001; c. 10.
Fig.4 CV(A) and EIS(B) results of the electrodes in different modification steps in 5.0 mmol/L K3[Fe(CN)6]/K4[Fe(CN)6] with a scan rate of 100 mV/s and in a scan range of -0.2—0.6 V a. Bare gold electrode; b. S2 immobilized on the electrode; c. S3 and S2 complementary paired on the electrode; d. S1 and S3 complementary paired on the electrode; e. adding 10 ng/mL AFB1 on the electrode.
Fig.5 Optimization of experimental parameters during the experiment (A) Concentration optimization of S3 binding to the electrode; (B) binding time of Cu-TPA/AuNPs/S1 to S3/S2/GE electrode; (C) combination time after the addition of AFB1 and S3. The concentration of AFB1 is 10 ng/mL.
Fig.6 DPV responses of the electrochemical biosensor to different concentrations of AFB1 under optimal experimental conditions(A) and the calibration curve between the peak current change and logarithm of AFB1 concentration(B) Concentration of AFBI/(ng·mL-1) from a to i: 10-6, 10-5, 10-4, 10-3, 10-2, 10-1, 1, 10, 100. The error bars in (B) are the standard deviations of the three experiments.
Analytical method | Material | Linear range/(ng·mL-1) | Detection limit/(ng·mL-1) | Ref. |
---|---|---|---|---|
Electrochemical immunosensor | AFB1-BSA/Cu-apatite | 10-3—100 | 2.0×10-4 | [ |
Electrochemiluminoscence | DNA2/HRP/AuNR | 5.0×10-6—10 | 4.3×10-7 | [ |
Electrochemiluminescence | luminol-AgNPs @MC | 10-4—50 | 5.0×10-5 | [ |
Electrochemicalsensors | MIP-based polymer/AuNPs | 10-6—1000 | 3.0×10-7 | [ |
Electrochemicalsensors | MIPOPD-Au/PtNP-MCNT | 10-4—10 | 3.1×10-5 | [ |
Electrochemical biosensor | Cu-TPA/AuNPs/S1 | 10-5—10 | 4.2×10-6 | This work |
Analytical method | Material | Linear range/(ng·mL-1) | Detection limit/(ng·mL-1) | Ref. |
---|---|---|---|---|
Electrochemical immunosensor | AFB1-BSA/Cu-apatite | 10-3—100 | 2.0×10-4 | [ |
Electrochemiluminoscence | DNA2/HRP/AuNR | 5.0×10-6—10 | 4.3×10-7 | [ |
Electrochemiluminescence | luminol-AgNPs @MC | 10-4—50 | 5.0×10-5 | [ |
Electrochemicalsensors | MIP-based polymer/AuNPs | 10-6—1000 | 3.0×10-7 | [ |
Electrochemicalsensors | MIPOPD-Au/PtNP-MCNT | 10-4—10 | 3.1×10-5 | [ |
Electrochemical biosensor | Cu-TPA/AuNPs/S1 | 10-5—10 | 4.2×10-6 | This work |
Spiked AFB1/(ng·mL-1) | Detected AFB1/(ng·mL-1) | Recovery(%) | RSD(%) |
---|---|---|---|
10 | 10.6 | 106 | 2.33 |
1 | 0.99 | 99 | 2.90 |
0.1 | 0.095 | 95 | 4.41 |
Spiked AFB1/(ng·mL-1) | Detected AFB1/(ng·mL-1) | Recovery(%) | RSD(%) |
---|---|---|---|
10 | 10.6 | 106 | 2.33 |
1 | 0.99 | 99 | 2.90 |
0.1 | 0.095 | 95 | 4.41 |
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