Interaction of Gold Nanoparticles with R-phycoerythrin

Abstract

Abstract:

Gold precursor solutions with pH values of 1, 5, 7, and 9 were prepared and the solutions were reduced to obtain colloid gold nanoparticles using NaBH₄ solution as reducing agent in presence of stabilizer. It is found that PVP, PVA, and Tween80 other than sodium dodecylbenzenesulfonate, PEG-1000, and OP emulsion are good stabilizers. When the colloid gold without any stabilizer has the maximum absorption at 525 nm, the absorption PVP-stabilized gold nanoparticles is shifted to 510 nm and the size of gold particles is about 5—8 nm. The interaction of gold nanoparticles with R-phycoerythrin was first investigated by UV-Vis absorption spectroscopy, X-ray fluorescence spectroscopy and transmission electron microscopy. It is found that the triple peak type of R-phycoerythrin at 498, 538 and 566 nm was observed. The average diameter of gold nanoparticles was about 10 nm and highly dispersed and stable in R-phycoerythrin solution. The intensity and position of the absorption spectrum at 538 nm was changed at the presence of gold nanoparticles and its intensive fluorescence emissions at 578 nm were decreased. It is suggested that the interaction between R-phycoerythrin and gold nanoparticles has an effect on the strcuture of R-phycoerythrin and can change the micro-environment of red-colored phycoerythrobilin chromophores in R-phycoerythrin. The results from gel-column chromatography and spectrophotometer analysis of R-phycoerythrin-gold colloid solution indicate that the interaction is also present, probably being resulted from amino groups included in R-phycoerythrin molecule.

Key words: Gold nanoparticle, R-phycoerythrin, Interaction

TrendMD:

JIN Ming-Shan, YUAN Hui-Qing, JING Ji-Rong, SUO Zhang-Huai*, SUN Li. Interaction of Gold Nanoparticles with R-phycoerythrin[J]. Chem. J. Chinese Universities, 2009, 30(6): 1183.

References

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