Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (6): 1116.

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Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate

LIAO Pei-Qiu^1,2, WEI Lai^1,2, LIU Wei³, WU Yi-Jie¹, LI Xiao-Jing¹*, NI Jia-Zuan¹, PEI Feng-Kui¹*

1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China;
3.Jilin Provincial Academy of Traditional Chinese Medicine, Changchun 130021, China

Received:2008-06-23 Online:2009-06-10 Published:2009-06-10
Contact: LI Xiao-Jing, E-mail: peifk@ciac.jl.cn; PEI Feng-Kui, E-mail: xjli@ciac.jl.cn
Supported by:
国家自然科学基金(批准号: 20575065和20637010)资助.

Abstract

Abstract:

The biochemical effects of neodymium nitrate were studied by NMR spectroscopic-based metabonomic approach. Male Wistar rats were treated intraperitoneally with different doses(2, 10 and 50 mg/kg body weight) of Nd(NO₃)₃. Liver samples from Nd³⁺-treated rats were analyzed either by 600 MHz ¹H MAS NMR techniques for intact liver or using high resolution(liquid state) ¹H NMR for liver chloroform/water/methanol extracts. All NMR spectra data were analyzed by pattern recognition using principal components analysis. Liver lesions induced by Nd(NO₃)₃ were represented by increased triglyceride, leucine(isoleucine), lactate, alanine, pyruvate, phosphocholine and glucose concentration, and reduced trimethylamine N-oxide(TMAO) concentration in liver. Histopathology examination showed cell necrosis and inflammation in the liver of 50 mg/kg body weight dosed rats. It was demonstrated that neodymium nitrate could cause damages to rats liver by disturbing the energy metabolism(glucose and lipid metabolism) and amino acid metabolism in the liver.

Key words: Nuclear magnetic resonance, Metabonomics, Pattern recognition, Liver, Neodymium nitrate, Biochemical effect

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LIAO Pei-Qiu, WEI Lai, LIU Wei, WU Yi-Jie, LI Xiao-Jing*, NI Jia-Zuan, ....... Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate[J]. Chem. J. Chinese Universities, 2009, 30(6): 1116.

References

[1]Lowe I.. Phys. Rev. Lett.[J], 1959, 2: 285—287
[2]Waters N. J., Holmes E., Williams A., et al.. Chem. Res. Toxicol.[J], 2001, 14: 1401—1412
[3]Wang Y. L., Bollard M. E., Keun H., et al.. Anal. Biochem.[J], 2003, 323: 26—32
[4]Waters N. J., Holmes E., Waterfield C. J., et al.. Biochem. Pharmacol.[J], 2002, 64: 67—77
[5]Garrod S., Humpher E., Connor S. C., et al.. Magn. Reson. Med.[J], 2001, 45: 781—790
[6]Griffin J. L., Walker L. A., Shore R. F., et al.. Xenobiotica[J], 2001, 31: 377—385
[7]Coen M., Lenz E. M., Nicholson J. K., et al.. Chem. Res. Toxicol.[J], 2003, 16: 295—303
[8]Kim H. K., Choi Y. H., Luijendijk T. J., et al.. Phytochem. Anal.[J], 2004, 15: 257—261
[9]Stentiford G. D., Viant M. R., Ward D. G., et al.. OMICS[J], 2005, 9: 281—299
[10]Shi C., Wu C. Q., Ca A. M., et al.. Toxicol. Lett.[J], 2007, 173: 161—167
[11]LIAO Pei-Qiu(廖沛球), WU Hui-Feng(吴惠丰), ZHANG Xiao-Yu(张晓宇), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2006, 27(8): 1448—1452
[12]Wu H. F., Zhang X. Y., Pei F. K., et al.. Anal. Biochem.[J], 2005, 339: 242—248
[13]LI Zhong-Feng(李中峰), WU Hui-Feng(吴惠丰), ZHANG Xiao-Yu(张晓宇), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2006, 27(3): 438—442
[14]Lin C. Y., Wu H. F., Tjeerdema R. S., et al.. Metabolomics[J], 2007, 3: 55—67
[15]Bligh E. G., Dyer W. J.. Can. J. Biochem. Physiol.[J], 1959, 37: 911—917
[16]Purohit P. V., Rocke D. M., Viant M. R., et al.. OMICS[J], 2004, 8: 118—130
[17]Garrod S., Bollard M. E., Nicholls A. W., et al.. Chem. Res. Toxicol.[J], 2005, 18: 115—122
[18]XU Lu(许禄), SHAO Xue-Guang(邵学广). Methods of Chemometrics(化学计量学方法)[M], Beijing: Science Press, 2004: 131—138
[19]LIAO Pei-Qiu(廖沛球), ZHANG Xiao-Yu(张晓宇), WEI Lai(魏来), et al.. Chinese J.Anal.Chem.(分析化学)[J], 2008, 36(4): 426—432

Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate

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