Immunonanogold Catalytic-Cu2O Particle Resonance Scattering Spectral Determination of Trace α-Fetoprotein

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (6): 1109.

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Immunonanogold Catalytic-Cu₂O Particle Resonance Scattering Spectral Determination of Trace α-Fetoprotein

JIANG Zhi-Liang¹*, ZHANG Yu-Lan¹, LIANG Ai-Hui², WEI Li-Li1, WANG Su-Mei²

1. Guangxi Key Laboratory of Enviromental Engineering, Protection and Assessment, School of Enviroment, Guangxi Normal University, Guilin 541004, China;
2. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China

Received:2008-10-10 Online:2009-06-10 Published:2009-06-10
Contact: JIANG Zhi-Liang, E-mail: zljiang@mailbox.gxnu.edu.cn
Supported by:
国家自然科学基金(批准号: 20667001, 20865002)、广西新世纪十百千人才资助计划基金和广西环境工程与保护评价重点实验室项目(批准号: 桂科能0702Z019)资助.

Abstract

Abstract:

15 nm-nanogold was used to label monoclone goat antihuman α-fetoprotein antibody(GAFP) to obtain immunonanogold probe(AuGAFP) for α-fetoprotein(AFP). Both nanogold and the probe have catalytic effect on the slow Cu₂O particle reaction between Fehling reagent and glucose that exhibit a resonance scattering peak at 620 nm. Combining AFP-AuGAFP immunoreaction with centrifugation technique, a highly sensitive immunonanogold catalytic-Cu₂O particle resonance scattering spectral assay for AFP was proposed. With addition of AFP, the AFP-AuGAFP immunocomplex increased, the excess probe in the supernatant decreased, and the resonance scattering intensity at 620 nm decreased linearly. The decreased intensity ΔI_RS was linear to AFP concentration[ρ(AFP)] in the range of 0.10—16.0 ng/mL, with a regression equation of ΔI_RS=4.27ρ(AFP)+1.28, and a detection limit of 0.05 ng/mL. This method was applied to the detection of AFP in sera, with high sensitivity and good selectivity, in addition to low-cost reagents and easy controlling reaction.

Key words: α-Fetoprotein, Nanocatalysis, Immunonanogold, Cu₂O particle, Resonance scattering spectral assay

TrendMD:

JIANG Zhi-Liang*, ZHANG Yu-Lan, LIANG Ai-Hui, WEI Li-Li, WANG Su-Mei. Immunonanogold Catalytic-Cu₂O Particle Resonance Scattering Spectral Determination of Trace α-Fetoprotein[J]. Chem. J. Chinese Universities, 2009, 30(6): 1109.

References

[1]Sun Y. L., Mi W., Cai J. Q., et al.. J. Am. Chem. Soc.[J], 2008, 130: 3847—3859
[2]Kew M. C., Dos S. H. A., Sherlock S.. Br. Med. J.[J], 1971, 4: 408—411
[3]CAO Dan-Ru(曹丹如), ZHENG Zuo-Ya(郑佐娅), ZHANG Jin-Feng(张锦锋), et al.. Lab. Med.(检验医学)[J], 2005, 20(3): 241—243
[4]Nakagawa T., Miyoshi E. J., Yakushijin T., et al.. J. Am. Chem. Soc.[J], 2008, 130(6): 2222—2233
[5]Helmut W., Felix G., Frank B.. Surgery[J], 2002, 131: 34—35
[6]Chuang L., Yang H. J., Chang H. C., et al.. Clin. Chim. Acta[J], 2004, 348: 87—93
[7]Ye Z. Q., Tan M. Q., Wang G. L., et al.. Talanta[J], 2005, 65: 206—210
[8]Ying F. C., Ran C. C., Yi J. L., et al.. Biosens. Bioelectron.[J], 2008, 23: 14—19
[9]Fu Z. F., Feng Y., Liu H., et al.. Biosens. Bioelectron.[J], 2008, 23: 1063—1069
[10]Liu Y. M., Mu H. B., Zheng Y. L., et al.. J. Chromatogr. B[J], 2007, 855: 280—285
[11]Zhang S. H., Zhang C., Xing Z., et al.. Clin. Chem.[J], 2004, 50: 1214—1221
[12]Wilson M. S., Nie W. Y.. Anal Chem.[J], 2006, 78: 6476—6483
[13]GAN Ning(干宁), HOU Lin-Xi(候琳熙), LI Tian-Hua(李天华), et al.. Amino Acids & Biotic Resources(氨基酸和生物资源)[J], 2007, 29(2): 73—75
[14]Holgate C. S., Jackson P., Cowen P. N., et al.. J. Histochem. Cytochem.[J], 1983, 31: 938—944
[15]Klara W., Christian S. M. A., Franz W.. Histochem. Cell Biol.[J], 2000, 114: 489—495
[16]CHU Xia(楚霞), XIANG Zhi-Feng(向知峰), SHEN Guo-Li(沈国励), et al.. J. Anal. Sci.(分析科学学报)[J], 2006, 22: 497—500
[17]Ma Z. F., Sui S. F.. Angew. Chem. Int. Ed.[J], 2002, 41: 2176—2179
[18]Mao X., Jiang J. H. , Luo Y., et al.. Talanta[J], 2007, 73: 420—424
[19]Du B. A., Li Z. P., Liu C. H.. Angew. Chem. Int. Ed.[J], 2006, 118: 8190—8193
[20]Liu S. P., Luo H. Q., Li N. B., et al.. Anal. Chem.[J], 2001, 73: 3907—3914
[21]Wu L. P., Li Y. F., Huang C. Z., et al.. Anal. Chem.[J], 2006, 78: 5570—5577
[22]XI Cun-Xian(郗存显), LIU Zhong-Fang(刘忠芳), LIU Shao-Pu(刘绍璞), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2008, 29(3): 510—514
[23]LU Qun-Min(鲁群岷), HE You-Qiu(何佑秋), LIU Shao-Pu(刘绍璞), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2006, 27(5): 849—852
[24]Jiang Z. L., Sun S. J., Liang A. H., et al.. Clin. Chem.[J], 2006, 52: 1389—1394
[25]Jiang Z. L., Sun S. J., Liang A. H., et al.. Anal. Chim. Acta[J], 2006, 571: 200—205
[26]Jiang Z. L., Liu S. P., Liu Q. Y.. Talanta[J], 2002, 58: 635—639
[27]Jiang Z. L., Huang G. X.. Clin. Chim. Acta[J], 2007, 376: 136—141
[28]Jiang Z. L., Huang W. X., Li J. P., et al.. Clin. Chem.[J], 2008, 54: 116—123
[29]Jiang Z. L., Huang Y. J., Liang A. H., et al.. Biosens. Bioelectron.[J], 2009, 24: 1674—1678

Immunonanogold Catalytic-Cu₂O Particle Resonance Scattering Spectral Determination of Trace α-Fetoprotein

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