Characterization of the Interaction Between Esterified TAM Radical and Bovine Serum Albumin

doi:10.7503/cjcu20170203

Abstract

Abstract:

Fully substituted tetrathiatriarylmethyl(TAM) radicals and their derivatives are ideal electron paramagnetic resonance(EPR) spectroscopy/imaging probe and have been received much concern in NMR contrast agent or spin traps fields. This work demonstrated that an esterified TAM radical(ET-03) bound to bovine serum albumin(BSA) in aqueous solution with fluorescence, electron paramagnetic resonance(EPR) spectroscopy, UV-Vis spectroscopy and molecular docking. The results showed that ET-03 spontaneously bound to BSA. The binding between ET-03 and BSA was driven by a hydrophobic interaction and the binding site was mainly determined to be the sub-domain ⅡA(Sudlow's site Ⅰ) and the sub-domain ⅢA(Sudlow's site Ⅱ). The fluorescence quenching of BSA by ET-03 was a mixture of static and dynamic mechanism with energy transfer probably. This protein binding of the esterified TAM probe to albumin can be used to study the structure and function of albumin and also must be considered for its use as an in vivo imaging agent or spin label.

Key words: Tetrathiatriarylmethyl radical, Ester derivative, Bovine serum albumin, Fluorescent quenching, Electron paramagnetic resonance(EPR) spectroscopy

CLC Number:

O625
O657

TrendMD:

AN Pengjiao, YU Nannan, SUN Ruisheng, SUI Xiaofang, SONG Yuguang. Characterization of the Interaction Between Esterified TAM Radical and Bovine Serum Albumin[J]. Chem. J. Chinese Universities, 2017, 38(8): 1354.

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