Chem. J. Chinese Universities ›› 2016, Vol. 37 ›› Issue (12): 2168.doi: 10.7503/cjcu20160500
• Organic Chemistry • Previous Articles Next Articles
LIU Lei1,2,3, LI Ke2,3,*(), HAO Xia1,2,3, WANG Guizhen1,2,3, QIN Xuemei2,3, DU Guanhua4, ZHANG Xiang5
Received:
2016-07-13
Online:
2016-12-10
Published:
2016-11-11
Contact:
LI Ke
E-mail:like@sxu.edu.cn
Supported by:
TrendMD:
LIU Lei, LI Ke, HAO Xia, WANG Guizhen, QIN Xuemei, DU Guanhua, ZHANG Xiang. Extraction, Separation, Structural Analysis and Antioxidant Activity in vitro of Arabinoxylans(AX-Ⅰ-1) from the Residue of Astragalus Root†[J]. Chem. J. Chinese Universities, 2016, 37(12): 2168.
Sugar | Methylated sugar | m/z | Molar ratio | Linkages type |
---|---|---|---|---|
Rha- | 2,3,4-Me3-Rha | 59, 72, 85, 102, 113, 118, 131, 162, 175 | 0.01 | L-Rhap-(1→ |
Ara- | 2-Me-Ara | 59, 74, 85, 99, 118, 127, 141, 159, 173, 187, 201, 217, 261 | 13.42 | →3,4)-L-Arap-(1→ |
Xyl- | 2-Me-Xyl | 61, 73, 86, 115, 128, 145, 159, 175, 188, 218, 243, 261, 289 | 8.13 | →2,3,4)-D-Xylp-(1→ |
Man- | 3,4,6-Me3-Man | 60, 71, 87, 99, 117, 129, 141, 161, 171, 189 | 0.10 | →2)-D-Manp-(1→ |
Glc- | 2,3,4,6-Me4-Glc | 59, 71, 87, 102, 118, 129, 145, 162, 175, 205 | 0.24 | D-Glcp-(1→ |
4-Me-Glc | 59, 71, 87, 100, 116, 129, 143, 160, 171, 189, 201, 262 | 0.23 | →2,3,6)-D-Glcp-(1→ | |
Gal- | 4,6-Me2-Gal | 59, 71, 86, 101, 112, 129, 143, 161, 188, 202 | 0.48 | →2,3)-D-Galp-(1→ |
3,4,6-Me3-Gal | 59, 71, 88, 100, 113, 119, 129, 145, 161, 174, 190 | 0.49 | →2)-D-Galp-(1→ |
Table 1 Partially O-methylated alditol acetates of AX-Ⅰ-1
Sugar | Methylated sugar | m/z | Molar ratio | Linkages type |
---|---|---|---|---|
Rha- | 2,3,4-Me3-Rha | 59, 72, 85, 102, 113, 118, 131, 162, 175 | 0.01 | L-Rhap-(1→ |
Ara- | 2-Me-Ara | 59, 74, 85, 99, 118, 127, 141, 159, 173, 187, 201, 217, 261 | 13.42 | →3,4)-L-Arap-(1→ |
Xyl- | 2-Me-Xyl | 61, 73, 86, 115, 128, 145, 159, 175, 188, 218, 243, 261, 289 | 8.13 | →2,3,4)-D-Xylp-(1→ |
Man- | 3,4,6-Me3-Man | 60, 71, 87, 99, 117, 129, 141, 161, 171, 189 | 0.10 | →2)-D-Manp-(1→ |
Glc- | 2,3,4,6-Me4-Glc | 59, 71, 87, 102, 118, 129, 145, 162, 175, 205 | 0.24 | D-Glcp-(1→ |
4-Me-Glc | 59, 71, 87, 100, 116, 129, 143, 160, 171, 189, 201, 262 | 0.23 | →2,3,6)-D-Glcp-(1→ | |
Gal- | 4,6-Me2-Gal | 59, 71, 86, 101, 112, 129, 143, 161, 188, 202 | 0.48 | →2,3)-D-Galp-(1→ |
3,4,6-Me3-Gal | 59, 71, 88, 100, 113, 119, 129, 145, 161, 174, 190 | 0.49 | →2)-D-Galp-(1→ |
Residue | δ | |||||
---|---|---|---|---|---|---|
1-H/C1 | 2-H/C2 | 3-H/C3 | 4-H/C4 | 5-H/C5 | 6-H/C6 | |
α-L-Rhap(1→ (A) | 5.02/102.4 | 4.34/70.9 | 3.99/81.0 | 3.44/72.0 | 3.83/69.5 | 1.24/ |
→3,4)β-L-Arap(1→(B) | 4.26/102.2 | 4.22/75.9 | 3.87/83.8 | 3.99/82.1 | 3.82/63.7 | -/- |
→2,3,4)β-D-Xylp(1→(C) | 4.76/107.0 | 3.07/74.4 | 3.44/73.1 | 3.82/81.6 | 3.36/59.6 | -/- |
→2)β-D-Manp(1→(D) | 4.91/94.6 | 3.36/80.9 | 3.25/75.9 | 3.87/73.1 | 3.82/70.0 | 3.71/63.7 |
β-D-Glcp(1→ (E) | 4.87/99.6 | 3.44/73.1 | 3.82/74.4 | 3.66/66.9 | 3.71/70.1 | 3.72/59.6 |
→2,3,6)α-D-Glcp(1→(F) | 5.01/99.8 | 3.42/81.0 | 3.66/73.1 | 3.71/75.4 | 3.82/70.0 | 3.87/73.1 |
→2,3)β-D-Galp(1→(G) | 4.84/102.4 | 3.82/69.3 | 3.99/82.6 | 4.04/70.0 | 3.66/75.9 | 3.71/59.6 |
→2)β-D-Galp(1→(H) | 4.82/102.0 | 3.72/70.0 | 3.87/73.6 | 3.89/71.8 | 3.44/74.8 | 3.72/60.5 |
Table 2 Chemical shifts for the resonances of glycosyl residues of AX-Ⅰ-1 in 1H and 13C NMR spectra
Residue | δ | |||||
---|---|---|---|---|---|---|
1-H/C1 | 2-H/C2 | 3-H/C3 | 4-H/C4 | 5-H/C5 | 6-H/C6 | |
α-L-Rhap(1→ (A) | 5.02/102.4 | 4.34/70.9 | 3.99/81.0 | 3.44/72.0 | 3.83/69.5 | 1.24/ |
→3,4)β-L-Arap(1→(B) | 4.26/102.2 | 4.22/75.9 | 3.87/83.8 | 3.99/82.1 | 3.82/63.7 | -/- |
→2,3,4)β-D-Xylp(1→(C) | 4.76/107.0 | 3.07/74.4 | 3.44/73.1 | 3.82/81.6 | 3.36/59.6 | -/- |
→2)β-D-Manp(1→(D) | 4.91/94.6 | 3.36/80.9 | 3.25/75.9 | 3.87/73.1 | 3.82/70.0 | 3.71/63.7 |
β-D-Glcp(1→ (E) | 4.87/99.6 | 3.44/73.1 | 3.82/74.4 | 3.66/66.9 | 3.71/70.1 | 3.72/59.6 |
→2,3,6)α-D-Glcp(1→(F) | 5.01/99.8 | 3.42/81.0 | 3.66/73.1 | 3.71/75.4 | 3.82/70.0 | 3.87/73.1 |
→2,3)β-D-Galp(1→(G) | 4.84/102.4 | 3.82/69.3 | 3.99/82.6 | 4.04/70.0 | 3.66/75.9 | 3.71/59.6 |
→2)β-D-Galp(1→(H) | 4.82/102.0 | 3.72/70.0 | 3.87/73.6 | 3.89/71.8 | 3.44/74.8 | 3.72/60.5 |
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