Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (5): 839.doi:

Previous Articles     Next Articles

Ordered Multilayer Film Electrode Containing Glucose Oxidase Based on Layer-by-layer Self-reaction

SUN Ying-Ying, ZHAO Shuang, YANG Wei-Wei, SUN Chang-Qing*   

  1. College of Chemistry, Jilin University, Changchun 130012, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10
  • Contact: SUN Chang-Qing

Abstract:

Highly stable glucose oxidase(GOx) multilayer films were fabricated on cystamine modified gold electrode surfaces by layer-by-layer self-reaction of periodate-oxidized glucose oxidase. The fabricated process was followed by electrochemical impedance spectroscopy. The experimental results showed that the film was homogeneous and formed in an ordered manner. The gold electrode modified with the GOx multilayers showed excellent electrocatalytical response to the oxidation of glucose when ferrocenemethanol was used as an artificial redox mediator. From the analysis of voltammetric signal, the coverage of active enzyme on the electrode surface was estimated, which had a linear relationship with the number of GOx besides the first layer. This suggested that the analytical performance such as sensitivity, detection limit, and so on, was tunable by controlling the number of the fabricated GOx layer. In addition, the biosensor exhibited a good reproducibility and stability.

Key words: Glucose oxidase; Biosensor; Layer-by-layer self-reaction; Ordered multilayer film; Electrocatalysis

CLC Number: 

TrendMD: