Recognition of Flavonoids Towards to DNA G-quadruplexes and Duplexes by ESI-MS

doi:10.3969/j.issn.0251-0790.2012.08.009

Abstract

Abstract: Electrospray ionization mass spectrometry(ESI-MS) was used for the investigation of noncovalent interactions between flavonoids, including rutin, quercetin, naringin, puerarin, and two types of DNA quadruplexes as well as three kinds of DNA duplexes. Percentage bound of each compound towards to different DNA strands were calculated based on the ion abundances of each DNA strand and DNA-ligand complexes obtained in the mass spectra. The results show that rutin and quercetin selectively bound to the G-quadruplexes, meanwhile, they also show strong binding to the duplexes. In contrast, naringin and puerarin show less selectivity towards to the G-quadrulexes.

Key words: Electrospray ionization mass spectrometry(ESI-MS), G-quadruplex DNA, Noncovalent interaction, Flavonoid

CLC Number:

O657.1

TrendMD:

LIU Zhen-Shuang, GAO Shang, ZHAO Wai-Ou, QIN Yu-Jiao, WANG Bing, GUO Xin-Hua. Recognition of Flavonoids Towards to DNA G-quadruplexes and Duplexes by ESI-MS[J]. Chem. J. Chinese Universities, 2012, 33(08): 1681.

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Recognition of Flavonoids Towards to DNA G-quadruplexes and Duplexes by ESI-MS

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