Chem. J. Chinese Universities

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Novel Electrochemical Sensor Based on Seminetwork-type Au Nanoparticles Labled Horseradish Peroxidase

LU Hai-Xia, WU Zai-Sheng, JIN Xiao-Yong, SHEN Guo-Li*, YU Ru-Qin   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, Changsha 410082, China
  • Received:2008-09-27 Revised:1900-01-01 Online:2009-02-10 Published:2009-02-10
  • Contact: SHEN Guo-Li

Abstract: A method of producing seminetwork-type Au nanoparticles labled by horseradish peroxidase(seminet-nanogold-HRP) with cross-linkers 1,6-hexananedithiol(HDT) to form seminet structure to increase the immobility of HRP was developed. Compared with HRP labled Au nanoparticles without HDT, the sensitive amperometric sensor of H2O2 mediated by seminet-nanogold-HRP, which was successfully immobilized on gold electrode supported by thiol-tailed groups of HDT, was more steady with a better current response in the low H2O2 concentration, and the detection limit reaches 0.08 μmol/L. Characterization of the seminet-nanogold-HRP was carried out using UV-Vis spectroscopy and TEM. The seminet-nanogold-HRP thin film was investigated by electrochemical impedence spectroscopy, quartz crystal microbalance and cyclic voltammograms.

Key words: Seminetwork-type Au nanoparticles labled by horseradish peroxidase(seminet-nanogold-HRP), Electrochemical sensor, Quartz crystal microbalance

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