Preparation and Application of Novel Amobarbital Electrochemical Sensor Based on CuO Nanoparticles Modified Glassy Carbon†

doi:10.7503/cjcu20140638

Abstract

Abstract:

A novel sensor is developed for the detection of amobarbital in urines, which is based on an electropolymerized molecularly imprinted polymer(MIP) on the surface of the CuO nanoparticles modified glassy carbon electrode. The optimums film formed conditions and experimental conditions were explored. The surface feature and performance of the modified electrode were characterized by scanning electron microscope(SEM), cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS). The results of electrochemical measurements indicated that CuO nanoparticles had good sensitization effected for the molecularly imprinted sensor and it exhibits good sensitivity and selectivity to the template molecule amobarbital. Under the optimal conditions, the relative redox peak currents of hexacyanoferrate were linear. The concentration of amobarbital ranged from 1.0×10^-7 to 1.4×10^-4 mol/L, with a linear correlation coefficient of 0.9966. The detection limit was 2.1×10^-9 mol/L(S/N=3). The prepared sensor was applied to the determination of amobarbital in urine samples with recovery ranging from 94.00% to 104.67%.

Key words: CuO nanoparticle, Amobarbital, Ethylene glycol maleic rosinate acrylate, Electrochemical sensor

CLC Number:

O657
O613

TrendMD:

HUANG Xueyi, YU Huicheng, WEI Yichun, LEI Fuhou, TAN Xuecai, WU Haiying. Preparation and Application of Novel Amobarbital Electrochemical Sensor Based on CuO Nanoparticles Modified Glassy Carbon^†[J]. Chem. J. Chinese Universities, 2014, 35(10): 2078.

Figures/Tables 9

Fig.1 Cyclic voltammograms for electropolymerization of imprinted film in the presence of AMB(A) and the nonimprinted membrance(B)

Fig.2 Effects of the elution time for MIP/CuO/GCE(a) and MIP/GCE(b) in 5.0 mmol/L K3[Fe(CN)6]-0.1 mol/L PBS

Fig.3 Effects of incubation time for the sensors CuO/MIP/GCE(A) and MIP/GCE(B) in 5.0 mmol/L K3[Fe(CN)6]-0.1 mol/L PBS (A) t/min, a—k: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11; (B) t/min, a—o: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15.

Fig.4 Cyclic voltammetries of different electrodes in 5.0×10-3 mol/L K3[Fe(CN)6]-0.1 mol/L PBS a. Bare GCE; b. MIP/CuO/GCE; c. MIP/GCE; d. MIP/CuO/GCE after interaction with 1.5×10-4mol/L AMB; e. NIP/CuO/GCE.

Fig.5 Electrochemical impedance spectra of different electrodes in 0.1 mol/L KCl-1.0×10-3 mol/L K3[Fe(CN)6]/K4[Fe(CN)6](1∶1, molar ratio) (A) a. Bare GCE; b. MIP/CuO/GCE; c. MIP/GCE; d. MIP/CuO/GCE after interaction with 5.0×10-5 mol/L AMB; e. NIP/CuO/GCE.

Fig.6 FESEM images of CuO modified bare GCE(A), and MIP/CuO/GCE electrodes before(B) and after(C) elution

Fig.7 Calibrations curve of AMB(A) and the differential pulse voltammetry incubation in different concentrations of AMB(B) in 5.0×10-3 mol/L K3[Fe(CN)6]-0.1 mol/L PBS (B) c/(μmol·L-1), a—j: 1.0×10-7, 5.0×10-7, 8.0×10-7, 2.0×10-6, 6.0×10-6, 1.5×10-5, 2.5×10-5, 5.0×10-5, 8.0×10-5, 1.4×10-4.

Fig.8 Determination results of interferences tested in 5.0×10-3 mol/L K3[Fe(CN)6]-0.1 mol/L PBS

Table 1 Determination results of recovery using the sensor

Sample	10⁵ Added/(mol·L^-1)	10⁵ Total found^* /(mol·L^-1)	Recovery(%)	RSD(%)
1	1.0	0.96	96.00	1.86
2	1.5	1.41	94.00	1.57
3	2.00	2.07	103.50	1.96
4	2.50	2.47	98.80	2.17
5	3.00	3.14	104.67	2.45

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