Single-walled Carbon Nanotube/DNAzyme Complex-based Elctrochemical Biosensors

doi:10.3969/j.issn.0251-0790.2012.08.008

Abstract

Abstract: Taking advantage of the excellent catalytic redox activity andthe electrochemical property of DNAzyme and carbon nanotubes, a novel nano-complex of single-walled carbon nanotubes(SWNTs)/DNAzyme was prepared, and the obtained nano-complex was finally immobilized on the surface of glass carbon electrode(GCE) with the assistance of chitosan to fabricate electrochemical biosensing interface. The catalytic property of the SWNTs/DNAzyme complex was explored, and a glucose oxidase biosensor was then prepared based on the fabricated electrochemical biosensing platform. The SWNTs/DNAzyme complex-based biosensing platform can direct response to hydrogen peroxide at low potential of -0.24 V with high sensitivity(the detection limit is 1?10^-6 mol/L) and wide linear range(5?10^-6-1?10^-2 mol/L), and the prepared glucose oxidase biosensor was used to fast and sensitive detection of glucose.

Key words: Carbon nanotube, DNAzyme, Electrochemical biosensor, Nano-complex, Catalytic property

CLC Number:

O657

TrendMD:

FENG Ke-Jun, YAO Yan-Ling, SHEN Guo-Li, YU Ru-Qin. Single-walled Carbon Nanotube/DNAzyme Complex-based Elctrochemical Biosensors[J]. Chem. J. Chinese Universities, 2012, 33(08): 1676.

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