Urinary Metabonomics Study of Type 2 Diabetic Rats Treated by Glimepiride

doi:10.7503/cjcu20120249

Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (10): 2169.doi: 10.7503/cjcu20120249

• Analytical Chemistry • Previous Articles Next Articles

Urinary Metabonomics Study of Type 2 Diabetic Rats Treated by Glimepiride

NIU Jun^1,2, PI Zi-Feng¹, YUE Hao³, LIU Shu-Ying^1,3

1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China;
3. Changchun University of Chinese Medicine, Changchun 130117, China

Received:2012-03-21 Online:2012-10-10 Published:2012-09-12

Abstract

Abstract:

A urinary metabonomics method based on rapid resolusion liquid chromatography-mass spectrometry(RRLC-MS) was developed to study the effect mechanism of glimepiride on type 2 diabetes mellitus. With multivariate statistical analysis of urinary metabolite profiles, a clear separation between streptozotocin-induced type 2 diabetic rats and those treated with glimepiride was achieved. Two significantly changed metabolites have been identified and used to explain the mechanism. The results show that the possible effect mechanism of glimepiride on type 2 diabetes is involved in amino acid metabolism.

Key words: Type 2 diabetic rat, Metabonomics, Rapid resolution liquid chromatography-mass spectrometry(RRLC-MS), Glimepiride

CLC Number:

O657.6

TrendMD:

NIU Jun, PI Zi-Feng, YUE Hao, LIU Shu-Ying. Urinary Metabonomics Study of Type 2 Diabetic Rats Treated by Glimepiride[J]. Chem. J. Chinese Universities, 2012, 33(10): 2169.

[1]	ZHANG Nanxi, LV Jingwei, JIN Ping, LI Jingfeng, BIAN Xuefeng, ZHANG Hui, SUN Jiaming. ¹H NMR Metabonomic Investigations of N-Benzylhexadecanamide Induced Proliferation and Iestosterone Secretion of Mouse Testicular Leydig Cells^† [J]. Chem. J. Chinese Universities, 2019, 40(9): 1832.
[2]	WANG Xiye, SHAN Xiaotong, WANG Yilin, LI Dan, ZHAO Ming, XU Liang. Mechanism of Salvia Miltiorrhiza on the Improvement of Myocardial Function in Patients with Dilated Cardiomyopathy^† [J]. Chem. J. Chinese Universities, 2016, 37(5): 844.
[3]	YANG Yongxia, WANG Linlin, ZHENG Lingyun, WANG Shumei, HUANG Rongbo, ZHANG Lei, HUANG Yaoting. Effects of Huanglian Jiedu Decoction on the Metabolic Features of Brown Adipose Tissue Extracts in Fructose-induced Insulin Resistance Model Based on NMR Metabonomics^† [J]. Chem. J. Chinese Universities, 2014, 35(9): 1883.
[4]	LIU Yue-Qin, PI Zi-Feng, SONG Feng-Rui, LIU Zhi-Qiang, LIU Zhong-Ying. Effect of Acarbose on Urine Metabolic Profiling of Type Ⅱ Diabete Rats [J]. Chem. J. Chinese Universities, 2012, 33(09): 1932.
[5]	LI Ying-Shuai, WANG Qi*, YUAN Zhuo-Jun. NMR-based Metabonomics Studies on Serum and Urine of Yang-deficiency Constitution [J]. Chem. J. Chinese Universities, 2011, 32(11): 2521.
[6]	WANG Wei-Hua, LIU Chun-Hong, SUN Yuan-Ming, LIN Feng, ZHANG Ming-Ming, WANG Zhao-Bin, ZHANG Min-Yi. Metabolic Profiles of Nonylphenol in Rat Urine [J]. Chem. J. Chinese Universities, 2011, 32(10): 2280.
[7]	LIANG Xiao-Ping, ZHANG Zheng, HU Ping, ZHONG Hong-Fu, GE Zhao-Hui, ZOU Ting-Ting, LIANG Qiong-Lin, WANG Yi-Ming, LUO Guo-An*. Metabonomics Study on the Acute Toxicity of Toad Venom [J]. Chem. J. Chinese Universities, 2011, 32(1): 38.
[8]	LIU Xia, XIAO Ying, GAO Hong-Chang, REN Jin, LIN Dong-Hai*. Metabonomic Study of Aristolochic Acid I-induced Acute Renal Toxicity Urine at Female and Male C57BL/6J Mice Based on ¹H NMR [J]. Chem. J. Chinese Universities, 2010, 31(5): 927.
[9]	WEI Yang-Yang, WANG Cai-Hong, LI Wei, DONG Ji-Yang*, CHEN Zhong. NMR-based Metabonomics Study on Serum and Urine of Hyperthyroidism [J]. Chem. J. Chinese Universities, 2010, 31(2): 279.
[10]	WANG Yong, JIANG Zhi-Ting, LIANG Qiong-Lin, WANG Yi-Ming, WANG Mei, .... Metabonomics Research of the Influence of Melamine to the Urine Metabolism of the Children Based on UPLC/TOF-MS [J]. Chem. J. Chinese Universities, 2010, 31(1): 57.
[11]	HAN Li-Da, LIANG Qiong-Lin, WANG Yi-Ming, LUO Guo-An*. Serum EFAs and NEFAs Metabolic Profiling Analysis Child-bearing Age Women and Its Application to NTDs Preventive Nutrition Intervention [J]. Chem. J. Chinese Universities, 2010, 31(1): 46.
[12]	DONG Ji-Yang, XU Le, XU Jing-Jing, CHEN Zhong. Adaptive Binning Method for NMR Spectroscopic Metabonomics Data Preprocessing [J]. Chem. J. Chinese Universities, 2009, 30(6): 1101.
[13]	LIAO Pei-Qiu, WEI Lai, LIU Wei, WU Yi-Jie, LI Xiao-Jing*, NI Jia-Zuan, ....... Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate [J]. Chem. J. Chinese Universities, 2009, 30(6): 1116.
[14]	HUANG Xin, GONG Yi-Fei, WANG Yi*, QU Hai-Bin, CHENG Yi-Yu. Metabonomic Study of Hepato-protective Effect of Silybin for the Carbon Tetrachloride Induced Liver Injure in Mice [J]. Chem. J. Chinese Universities, 2008, 29(4): 714.
[15]	ZHANG Jie, YAN Li-Juan, LIN Lin, CHEN Wen-Gui, SONG Xiu-Yu, YAN Xiao-Mei, HANG Wei, HUANG Ben-Li. Metabonomics Research of Diabetes Mellitus and Diabetic Nephropathy Based on UPLC-oaTOF-MS System [J]. Chem. J. Chinese Universities, 2008, 29(11): 2171.

Urinary Metabonomics Study of Type 2 Diabetic Rats Treated by Glimepiride

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments