Abstract:

Acomplex sulfated fucoidan (SPF) was obtained by hot water extraction from Sargassum pallidum. A series of hybrid oligosaccharides were acquired by mild acid hydrolysis with low pressure gel permeation chromatography(LPGPC). Based on the monosaccharides composition, methylation and electrospray collision-induced dissociation mass spectrometry(ES-CID-MS/MS) analysis, 21 kinds of oligosaccharides were sulfated hybrid fucoidan-derived oligosaccharides. The resulted oligosaccharides were mainly composed of α1→3 linked Fuc, β1→4 linked Xyl and β1→6 linked Gal. The sulfation occurred at C4 or C2 of fucose, C2 of xylose and C4 of galactose residue, respectively. Most of fucose residues were present in nonreducing terminal of oligosaccharides. The technique of ES-CID-MS/MS was suitable for the sequences analysis of other hybrid oligosaccharides of various fucoidans. More importantly, all these diverse sulfated oligosaccharides can be printed onto the glycol-chip, and studying their interactions with proteins.

Key words: Sargassum pallidum, Fucoidan, Oligosaccharide, Electrospray collision-induced dissociation mass spectrometry(ES-CID-MS/MS)