Determination of Eugenol in Several Chinese Herbal Medicines by Capillary Electrophoresis with High Frequency Conductivity Method

Chem. J. Chinese Universities

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Determination of Eugenol in Several Chinese Herbal Medicines by Capillary Electrophoresis with High Frequency Conductivity Method

LIN Wei-Feng¹, WU Xiao-Lin², CHEN Zuan-Guang³, LI Quan-Wen³, CAI Pei-Xiang¹, MO Jin-Yuan¹

1. School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China;
2. The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China;
3. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510089, China

Received:2006-04-13 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10

Abstract

Abstract: A method based on capillary electrophoresis with a high frequency conductivity method was developed for the determination of eugenol in several Chinese herbal medicines from different producing areas. In this study, some key factors for separation and detection were studied and the best analysis conditions were obtained. In the buffer system of 2.0 mmol/L triethylamine +0.74 mol/L methanol at separation potential of 18.0 kV, the optimal separation and detection wasobtained. Under the chosen conditions, eugenol was eluted within 7 min. The linear range of eugenol was 2.0—540 μg/mL, the limit of detection was 0.6 μg/mL, and the recovery ranged from 93.3% to 106%.

Key words: Capillary electrophoresis, High frequency conductivity detection, Cont actless conductivity detection, Eugenol, Chinese herbal medicine

CLC Number:

O657

TrendMD:

LIN Wei-Feng¹, WU Xiao-Lin², CHEN Zuan-Guang³, LI Quan-Wen³, CAI Pei-Xiang¹, MO Jin-Yuan¹. Determination of Eugenol in Several Chinese Herbal Medicines by Capillary Electrophoresis with High Frequency Conductivity Method[J]. Chem. J. Chinese Universities, doi: .

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Determination of Eugenol in Several Chinese Herbal Medicines by Capillary Electrophoresis with High Frequency Conductivity Method

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