Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry

Abstract

Abstract: Metabolism of pilocarpine in human urine was studied by high-performance liquid chromatography with tandem mass spectrometric method after single oral dose 10 mg pilocarpine hydrochloride to a healthy male volunteer.The urine sample was collected from 0 to 8 h after oral administration. The urine sample was prepared by solid phase extraction,and then was analyzed using high-performance liquid chromatography coupled with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring and product ion scan mode. Three metabolites were identified as the oxidative metabolite,pilocarpic acid and the glucuronide of pilocarpine.

Key words: Pilocarpine; Metabolite; Liquid chromatography-tandem mass spectrometry, Pilocarpine, Metabolite, Liquid chromatography-tandem mass spectrometry

CLC Number:

Q516

TrendMD:

WANG Ling, WANG Ying-Wu, CHEN Gang, YUAN Hong-Ming. Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry[J]. Chem. J. Chinese Universities, 2005, 26(12): 2230.

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Studies on Metabolites of Pilocarpine in Human Urine Using High-performance Liquid Chromatography with Tandem Mass Spectrometry

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