Abstract: A series of Morin-imprinted polymers were prepared and their adsorption properties for the template were evaluated by binding experiments.The results show that the polymer prepared by using acrylamide as the functional monomer,CH₃CO₂Et as the porogen exhibited a higher selectivity for Morin and good affinity to its structural analogues.The molar ratio of template to functional monomer in the pre-polymeric mixture was optimized.Scatchard analysis shows that a equal class of binding sites were formed in the imprinted polymer in the studied concentration range.The dissociation constant and the apparent maximum number of the binding sites were 0.877 mmol/L and 59.18 μmol/g,respectively.The function of the functional groups of the template in the formation of complementary interacting site in the polymer was discussed.It was concluded that the hydrogen bonds formed between the C-₃—OH,C-₇—OH,C-₄'—OH of Morin and acrylamide played an important role in molecular recognition.Using the Morin-imprinted polymers as thin layer chromatographic stationary phase can separate the template from the other compounds.The minimal value of R-s to its structural analogue compound was 1.19.

Key words: Molecular imprinting, Morin, Acrylamide, Substrate selectivity, Thin layer chromatography