Immobilization of Cellulase Based on Polyvinyl Alcohol/Fe2O3 Nanoparticles

Chem. J. Chinese Universities

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Immobilization of Cellulase Based on Polyvinyl Alcohol/Fe₂O₃Nanoparticles

LIAO Hong-Dong, YUAN Li, TONG Chun-Yi, ZHU Yong-Hua, LI Du, LIU Xuan-Ming*

Bioenergy and Biomaterial Reseasch Center, Institute of Life Science and Biotechnology, Hunan University, Changsha 410082, China

Received:2008-01-21 Revised:1900-01-01 Online:2008-08-10 Published:2008-08-10
Contact: LIU Xuan-Ming

Abstract

Abstract: To improve the enzyme activity and reusability, a new soluble, easy separated carrier was made to immolize cellulase. The immobilized cellulase based on polyvinyl alcohol/Fe₂O₃ nanoparticles was prepared with cyclic freezing thawing process. Analytic data from TEM, IR and VSM detection suggest that the average diameters of immobilized cellulase complexs were 1 μm, which contained about 10 nm Fe₂O₃ naoparticle. The factors affecting the activities of immobilized cellulase were researched. The activity retention of immobilized cellulase was achieved as 42% under the optimum conditions: pH=6.0, mass ratio of cellulase to PVA is equal to 4, mass ratio of PVA to Fe is equal to 50, 11 h for immobilization. The immobilized cellulase exhibited greater efficiency than free one and retained 50% relative activity after five times of cycled reuse, which indicates that this novel method of immobilization could be propitious to cellulase reuse and efficiency improvement.

Key words: Cellulase, Immobilizatoin, Polyvinyl alcohol, Fe₂O₃ naoparticle

CLC Number:

Q595

TrendMD:

LIAO Hong-Dong, YUAN Li, TONG Chun-Yi, ZHU Yong-Hua, LI Du, LIU Xuan-Ming*. Immobilization of Cellulase Based on Polyvinyl Alcohol/Fe₂O₃Nanoparticles[J]. Chem. J. Chinese Universities, doi: .

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Immobilization of Cellulase Based on Polyvinyl Alcohol/Fe₂O₃Nanoparticles

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