A Novel Polyamide/Ser-His Conjugate with DNA-cleaving Function

Chem. J. Chinese Universities

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A Novel Polyamide/Ser-His Conjugate with DNA-cleaving Function

NIU Ming-Yu¹; YE Yong²; LIU Wei¹; ZHANG Zhen¹; ZHAO Yu-Fen^1,2*

1. Key laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology,
Ministry of Education, Department of Chemistry, Tsinghua University,
Beijing 100084, China;
2. Department of Chemistry, Key Laboratory of Chemical Biology and Organic
Chemistry, Zhengzhou University, Zhengzhou 450052, China

Received:2005-06-07 Revised:1900-01-01 Online:2006-07-10 Published:2006-07-10
Contact: ZHAO Yu-Fen

Abstract

Abstract: Recently, polyamides containing N-methylpyrrole have attracted considerable attention from synthetic and biological chemists. Based on Dervan's theory, the polyamide/SerHis conjugate which is a synthetic analogue of natural products for DNA affinity was synthesized through the dicyclohexylcarbodiimide/1-hydroxybenzotriazole(DCC/HOBt) coupling reaction, and its cleavage activity for DNA was also studied. In BR buffer(pH=60), pBR322 DNA can be cleavaged by the title compound at 37 ℃. After 6 h, pBR 322 DNA was transferred from form Ⅰ to form Ⅱ, after 36 h, pBR 322 DNA was cleavaged totally.

Key words: Polyamide/Ser-His conjugate, DNA, Cleavage agen

CLC Number:

O321.3⁺3

TrendMD:

NIU Ming-Yu¹; YE Yong²; LIU Wei¹; ZHANG Zhen¹; ZHAO Yu-Fen^1,2*. A Novel Polyamide/Ser-His Conjugate with DNA-cleaving Function[J]. Chem. J. Chinese Universities, doi: .

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A Novel Polyamide/Ser-His Conjugate with DNA-cleaving Function

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