Optimized Separation and Identification of Proteome from the Baccal Ganalion of Aplysia(Notarcus leachii cirrosus Stimpson)

Chem. J. Chinese Universities

• 研究论文 • Previous Articles Next Articles

Optimized Separation and Identification of Proteome from the Baccal
Ganalion of Aplysia(Notarcus leachii cirrosus Stimpson)

CHEN Dong-Shi¹; HUANG He-Qing^1,2,3*; WU Han-Zhi^1,3; CAI Zong-Wei^3,4

1. The Key Laboratory of MOE for Cell Biology and Tumor Cell Engineering,
School of Life Science,
2. State Key Laboratory of Marine Environmental Science,
3. State Key Laboratory for Physical Chemistry of the Solid Surface, Xiamen
University, Xiamen 361005, China;
4. Department of Chemistry, Hong Kong Baptist University, Hong Kong, China

Received:2005-06-13 Revised:1900-01-01 Online:2006-07-10 Published:2006-07-10
Contact: HUANG He-Qing

Abstract

Abstract: A separation method of proteome from the buccal ganglion of aplysia(Notarcus leachii cirrosus Stimpson, NLCS) with two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) was optimized, gaining about 300 protein spots. Mass spectrogram of peptide mass fingerprint(PMF) of 96 protein spots from the BG were obtained by a combined off-line technique of the matrix-assisted laser desorption ionization mass spectrometry(MALDI-TOF MS) and the emzymolysis in gel. Based on the identification via the databases searches and PMF maps, we found that four of 96 proteins in the gel showed the characteristics with high match scores, which were indicated to be framework proteins tubulin, actin, ribulose-1,5-bisphosphate carboxylase and oxygenase in neurons cell; meanwhile, a peptide mating pheromone precursor was found. Fifty-six proteins in BG were further classified by a LOC tree software according to the search result of subcellular localization.

Key words: Aplysia, Buccal ganglion, Proteome, 2D-PAGE, Mass spectrometry

CLC Number:

TrendMD:

CHEN Dong-Shi¹; HUANG He-Qing^1,2,3*; WU Han-Zhi^1,3; CAI Zong-Wei^3,4. Optimized Separation and Identification of Proteome from the Baccal
Ganalion of Aplysia(Notarcus leachii cirrosus Stimpson)[J]. Chem. J. Chinese Universities, doi: .

[1]	ZHU Kai, LI Jie, WU Xiaoyi, HU Weiwei, WU Dongmei, YU Chengxiao, GE Zhiwei, YE Xingqian, CHEN Shiguo. Combined PGC-Triple-Tof-MS Enables the Separation， Identification of Sugar Beet Pectin Derived Oligomers [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220023.
[2]	LIANG Quntao, ZOU Qiang, LIN Jianghui, LIU Shutao, WEI Zheng. Ultraviolet Absorbance of Saturated Structure in the Non-reducing End of Heparin/Heparan Sulfate Disaccharides and Its Application in Sequence of Heparin Oligosaccharides [J]. Chem. J. Chinese Universities, 2021, 42(6): 1776.
[3]	DOU Shuzhen, WANG Zhongshun, LYU Nan. Improving the Detection Performance of Surface-assisted Laser Desorption/ionization Mass Spectrometry by Silicon Nanostructures [J]. Chem. J. Chinese Universities, 2021, 42(4): 1156.
[4]	ZHENG Haijiao, JIANG Liyan, JIA Qiong. Arginine Functionalized Magnetic Nanoparticles and Its Application in Phosphopeptides Enrichment [J]. Chem. J. Chinese Universities, 2021, 42(3): 717.
[5]	ZHAO Huanhuan, LI Cunyu, JIN Hong. Evaluation of Quantitative Phosphoproteomic Performance of Sequential Enrichment of Metal Oxide Affinity Chromatography for Phosphopeptides Using TMT 10-plex Isobaric Labeling [J]. Chem. J. Chinese Universities, 2021, 42(12): 3624.
[6]	WANG Ruxin, ZHAO Zhongjun, HE Feiyao, YUE Hanlu, DENG Fulong, LI Hong, LI Wenwen, DUAN Yixiang. Characteristic Analysis of C₁—C₃n-Aldehydes and n-Alcohols in Proton Transfer Reaction Time-of-flight Mass Spectrometry [J]. Chem. J. Chinese Universities, 2021, 42(12): 3632.
[7]	WANG Baichun, YUAN Yuxin, YAN Yinghua, DING Chuanfan, TANG Keqi. Glucose-6-phosphate Functionalized Hydrophilic Magnetic Probe： a Dual-purpose Affinity Material for Effective Separation and Enrichment of Glycopeptides/Phosphopeptides [J]. Chem. J. Chinese Universities, 2021, 42(10): 3062.
[8]	DENG Jiewei, YANG Yunyun, LIN Li, LUAN Tiangang. Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics [J]. Chem. J. Chinese Universities, 2020, 41(9): 2011.
[9]	LI Xiaoqian, ZHANG Hua, LU Haijian, LIU Chang, LIU Qinglong, MA Xiayu, FANG Yuanping, LIANG Dapeng. Mechanism of Photocatalytic Degradation of Rhodamine B by TiO₂ Nanowire Array with Internal Extraction Electrospray Ionization Mass Spectrometry [J]. Chem. J. Chinese Universities, 2020, 41(9): 2003.
[10]	WANG Xiaoyu, YAN Guoquan, ZHOU Xinwen, YANG Pengyuan. Assignment of Polypeptide Disulfide Bonds by Chemical Cleavage and Bio-mass Spectrometry^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1505.
[11]	HE Yu, XIA Qian, WANG Wenli, LUO Fang, CHEN Jinfeng, GUO Yating, WANG Jian, LIN Zhenyu, CHEN Guonan. Triglycerides as Potential Biomarkers for Pesticides Pollution in Aquatic Environment Using UPLC-QTOF-MS ^† [J]. Chem. J. Chinese Universities, 2020, 41(3): 431.
[12]	WANG Tianqi,YU Qiongwei,FENG Yuqi. Analysis of Imidazole Propionic Acid in Serum of Patients with Type 2 Diabetes Based on NiO@SiO₂ Solid-phase Extraction Coupled with Liquid Chromatography-Mass Spectrometry ^† [J]. Chem. J. Chinese Universities, 2020, 41(2): 262.
[13]	AI Wanpeng, SONG Shiyao, BAI Yu, LIU Huwei. Development and Applications of Mass Spectrometry in Reaction Monitoring [J]. Chem. J. Chinese Universities, 2020, 41(12): 2598.
[14]	WANG Xiaoqun, HUANG Guangming. Investigation of Lipid Distributions by Trifluoroacetic Acid-enhanced Desorption Electrospray Ionization Mass Spectrometry Imaging [J]. Chem. J. Chinese Universities, 2020, 41(12): 2673.
[15]	WANG Lianping,LI Qingjie,LIU Xiaoyan,REN Yueying,YANG Xiuwei. Screening of Cholinesterase Inhibitors in Fructus Evodiae Alkaloids Based on UFLC-MS/molecular Simulation ^† [J]. Chem. J. Chinese Universities, 2020, 41(1): 111.

Optimized Separation and Identification of Proteome from the Baccal
Ganalion of Aplysia(Notarcus leachii cirrosus Stimpson)

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments