Isolation and Purification and Structural Characterization of Acidic Polysaccharide FCP5-A from Fruit of Cornus officinalis

Chem. J. Chinese Universities

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Isolation and Purification and Structural Characterization of Acidic Polysaccharide FCP5-A from Fruit of Cornus officinalis

YANG Li-Yan¹, HUANG Lin-Juan¹, WANG Zhong-Fu¹*, CAO Chun-Yang², SUN Wen-Ji¹

1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key Laboratory of Biotechnology, Life Science College, Northwest University, Xi’an 710069, China;
2. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China

Received:2007-08-20 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10
Contact: WANG Zhong-Fu

Abstract

Abstract: Fruit of Cornus officinalis has been used in Chinese traditional medicine for over two thousand of years. Polysaccharides, as important components of Cornus officinalis, have attracted much attention. An acidic polysaccharide FCP5-A was isolated from fruit of Cornus officinalis with water extraction and column chromatography. HPGPC shows that it was homogeneous polysaccharide with an average molecular weigh of 8.7×10⁴. Monosaccharide analysis by GC shows that it was composed of rhamnose, arabinose, galactose and galacturonic acid with a molar ratio of 1∶ 5.7∶ 0.6∶ 1.2. By means of IR, GC, partial hydrolysis with acid, uronic acid reduction, methylation, GC-MS and ¹³C NMR analysis, the results indicate that FCP5-A was a branched polysaccharide rich in arabinose. The backbone was composed of 1,2-linked Rha and 1,4-linked GalA, with side chains attached to O-4 of the rhamnosyl residues. The side chains contained highly branched arabinan and short linear 1,3-linked galactan. It was concluded that FCP5-A was a new acidic branched polysaccharide of Cornus officinalis.

Key words: Cornus officinalis, Polysaccharide, Isolation and purification, Structure characterization

CLC Number:

O629

TrendMD:

YANG Li-Yan¹, HUANG Lin-Juan¹, WANG Zhong-Fu¹*, CAO Chun-Yang², SUN Wen-Ji¹. Isolation and Purification and Structural Characterization of Acidic Polysaccharide FCP5-A from Fruit of Cornus officinalis[J]. Chem. J. Chinese Universities, doi: .

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Isolation and Purification and Structural Characterization of Acidic Polysaccharide FCP5-A from Fruit of Cornus officinalis

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