Abstract: C₁₈ esterified silica monolithic column emerging from chromatogram realm was made by use of solid-phase extraction medium material in μ-TAS. Several simple solid-phase extraction pretreatment units were set up to preconcentrate dobutamine hydrochloride which was detected by dobutamine hydrochloride-KMnO₄-Luminol chemiluminescence system. Trace dobutamine hydrochloride in serum was enriched by C₁₈ esterified silica monolithic column, the average enrichment was 77.2 times, RSD was 12.35%. In order to overcome the shortage of conformable precision, polyamide affinity membrane modified by cibacron blue F3GA was introduced into the pretreatment unit to get rid of interferer from large molecule such as albumin in serum. The affinity membrane was combined with micro-fluidic chip by embedded and external methods and using external-combination pretreatment unit, average enrichment was 89.36 times, RSD was 4.37%. The result indicate the feasibility and validity of the pretreatment unit for trace medicine enrich in serum.

Key words: Analytical system with micro-fluidic chip, Solid-phase extraction pretreatment unit, Silica monolithic column, Affinity membrane