Chem. J. Chinese Universities ›› 2018, Vol. 39 ›› Issue (1): 32.doi: 10.7503/cjcu20170539
• Analytical Chemistry • Previous Articles Next Articles
WANG Li, LI Zhi, SHEN Xiaoqin, MA Nan*()
Received:
2017-08-07
Online:
2018-01-10
Published:
2017-12-06
Contact:
MA Nan
E-mail:nan.ma@suda.edu.cn
Supported by:
CLC Number:
TrendMD:
WANG Li, LI Zhi, SHEN Xiaoqin, MA Nan. Programming Single Quantum Dot Valencies via DNA Caging†[J]. Chem. J. Chinese Universities, 2018, 39(1): 32.
Name | DNA sequence(5'-3') |
---|---|
DNA1 | TCGCTGAGTAATCGTAACTCGCCTACTGGTCAGCAAGTGTGGGCACGCACACAGTAGTAATACCAGAT-GGAGTACACAAATCTG |
DNA2 | CTATCGGTAGAGCAGCTATGGATCCGACGTTATACTCAGCGACAGATTTGTGAGATGACCTCTGATTA-CGCGTACAACTAGCGG |
DNA3 | CACTGGTCAGATACAGAATCACTGGAACAGTACTACCGATAGCCGCTAGTTGAGATGCCTAAGTA-GCGCCATGAGGTTTGCTGA |
DNA4 | CCACACTTGCAAGTCCTAGACGGTAGCTCGAACTGACCAGTGTCAGCAAACCAGACTA-TCGATGGCATAATCCAGTGTGCGTGC |
DNA5 | GTACGCGTCTAGGATCAAAAAGTTCCAGTGATTCTGTA |
DNA6 | TCGAGCTACCGTCTAGGACT |
DNA7 | GACCAGTAGGCGAGTTACGA |
DNA8 | AACGTCGGATCCATAGCTGC |
DNA9 | GGATTATGCCATCGATAGTC |
DNA10 | CATGGCGCTACTTAGGCATC |
DNA11 | ACGCGTAATCAGAGGTCATC |
DNA12 | ACTCCATCTGGTATTACTACAAAAAAAAAAAA |
DNA13(ps-po DNA) | GATCCTAGACGCGTACAAAAAG*G*G*G*G*G*G*G*G*G*TTTTTTTTTTTT |
DNA14(thiolated) | CATTGATCCGAGCCTAAAAAAAAAA |
DNA15(thiolated) | TGATTCCAGGTAGCAAAAAAAAAAA |
DNA16(thiolated) | CGTGCTAAGTGCGATAAAAAAAAAA |
DNA6' | TGCTACCTGGAATCATCGAGCTACCGTCTAGGACT |
DNA7' | AGGCTCGGATCAATGGACCAGTAGGCGAGTTACGA |
DNA11' | ATCGCACTTAGCACGACGCGTAATCAGAGGTCATC |
DNA12'(Cy-5) | ACTCCATCTGGTATTACTACAAAAAAAAAAAA-Cy5 |
Table 1 DNA sequences
Name | DNA sequence(5'-3') |
---|---|
DNA1 | TCGCTGAGTAATCGTAACTCGCCTACTGGTCAGCAAGTGTGGGCACGCACACAGTAGTAATACCAGAT-GGAGTACACAAATCTG |
DNA2 | CTATCGGTAGAGCAGCTATGGATCCGACGTTATACTCAGCGACAGATTTGTGAGATGACCTCTGATTA-CGCGTACAACTAGCGG |
DNA3 | CACTGGTCAGATACAGAATCACTGGAACAGTACTACCGATAGCCGCTAGTTGAGATGCCTAAGTA-GCGCCATGAGGTTTGCTGA |
DNA4 | CCACACTTGCAAGTCCTAGACGGTAGCTCGAACTGACCAGTGTCAGCAAACCAGACTA-TCGATGGCATAATCCAGTGTGCGTGC |
DNA5 | GTACGCGTCTAGGATCAAAAAGTTCCAGTGATTCTGTA |
DNA6 | TCGAGCTACCGTCTAGGACT |
DNA7 | GACCAGTAGGCGAGTTACGA |
DNA8 | AACGTCGGATCCATAGCTGC |
DNA9 | GGATTATGCCATCGATAGTC |
DNA10 | CATGGCGCTACTTAGGCATC |
DNA11 | ACGCGTAATCAGAGGTCATC |
DNA12 | ACTCCATCTGGTATTACTACAAAAAAAAAAAA |
DNA13(ps-po DNA) | GATCCTAGACGCGTACAAAAAG*G*G*G*G*G*G*G*G*G*TTTTTTTTTTTT |
DNA14(thiolated) | CATTGATCCGAGCCTAAAAAAAAAA |
DNA15(thiolated) | TGATTCCAGGTAGCAAAAAAAAAAA |
DNA16(thiolated) | CGTGCTAAGTGCGATAAAAAAAAAA |
DNA6' | TGCTACCTGGAATCATCGAGCTACCGTCTAGGACT |
DNA7' | AGGCTCGGATCAATGGACCAGTAGGCGAGTTACGA |
DNA11' | ATCGCACTTAGCACGACGCGTAATCAGAGGTCATC |
DNA12'(Cy-5) | ACTCCATCTGGTATTACTACAAAAAAAAAAAA-Cy5 |
Size/nm | V(H2O)/mL | V(HAuCl4)/μL | V(Citrate acid)/mL |
---|---|---|---|
15 | 25 | 250 | 1.25 |
20 | 30 | 300 | 1.05 |
Table 2 Reactant volumes of gold nanoparticles with different sizes
Size/nm | V(H2O)/mL | V(HAuCl4)/μL | V(Citrate acid)/mL |
---|---|---|---|
15 | 25 | 250 | 1.25 |
20 | 30 | 300 | 1.05 |
Fig.3 Absorption(a) and photoluminescence(b) spectra of DNA13-QD(A), native PAGE characterization of hybridization between DNA13-QD(lane 1) and DNA5(lane 2)(B) and low(C) and high-resolution(D) TEM images of DNA13-QD
Fig.4 Native PAGE characterization of heterobivalent QD(lane 1), DNA cube-QD(lane 2), and DNA cube(lane 3), low magnification(B1) and high-resolution(B2) TEM images of DNA cube-QD stained with phosphotungstic acid and AFM image(C) and height statistics(D) of DNA cube-QDThe DNA cube was pre-stained with GelRed before loading on the gel.
Fig.5 Fluorescence spectra of DNA cube-QD(a), DNA cube-QD with Cy5 labeling(b) and empty DNA cube with Cy5 labeling(c)(A) and QD-Cy5(a), QD(b), QD-Cy5+cDNA(c)(B)All samples were excited at 405 nm.
Fig.6 Schematic illustration of DNA-caged QD with adjacent(1 and 2) and diagonal(2 and 3) valencies(A), low and high magnification TEM images of GNPs assembled to bivalent DNA-caged QD with adjacent valencies(B) and diagonal valencies(C)
Fig.7 Statistics of the separation distance(center to center, inset) of GNPs conjugated to bivalent QDs with adjacent(A) and diagonal valencies(B)50 complex structures were examined in each case.
Fig.8 Assembly of different sized GNPs with DNA-caged QDs(A) Low and high magnification TEM images of small and large GNPs assembled to bivalent DNA-caged QD with adjacent position; (B) low and high magnification TEM images of small, medium and large GNPs assembled to trivalent DNA-caged QD with adjacent and diagonal position.
[1] | Michalet X., Pinaud F. F., Bentolila L. A., Tsay J. M., Doose S., Li J. J., Sundaresan G., Wu A. M., Gambhir S. S., Weiss S., Science, 2005, 307(5709), 538—544 |
[2] | Medintz I. L., Uyeda H. T., Goldman E. R., Mattoussi H., Nat. Mater., 2005, 4(6), 435—446 |
[3] | Bruchez M. J., Moronne M., Gin P., Weiss S., Alivisatos A. P., Science, 1998, 281(5385), 2013—2016 |
[4] | Gao X., Cui Y., Levenson R. M., Chung L. W., Nie S., Nat. Biotechnol., 2004, 22(8), 969—976 |
[5] | Liu W., Howarth M., Greytak A. B., Zheng Y., Nocera D. G., Ting A. Y., Bawendi M. G., J. Am. Chem. Soc., 2008, 130(4), 1274—1284 |
[6] | Yu J. H., Kwon S. H., Petrášek Z., Park O. K., Jun S. W., Shin K., Choi M., Park Y. I., Park K., Na H. B., Lee N., Lee D. W., Kim J. H., Schwille P., Hyeon T., Nat. Mater., 2013, 12(4), 359—366 |
[7] | Biju V., Itoh T., Ishikawa M., Chem. Soc. Rev., 2010, 39(8), 3031—3056 |
[8] | Pons T., Medintz I. L., Wang X., English D. S., Mattoussi H., J. Am. Chem. Soc., 2006, 128(47), 15324—15331 |
[9] | Lee H., Lytton-Jean A. K., Chen Y., Love K. T., Park A. I., Karagiannis E. D., Sehgal A., Querbes W., Zurenko C. S., Jayaraman M., Peng C. G., Charisse K., Borodovsky A., Manoharan M., Donahoe J., Truelove J., Nahrendorf M., Langer R., Anderson D. G., Nat. Nanotechnol., 2012, 7(6), 389—393 |
[10] | Mirkin C. A., Letsinger R. L., Mucic R. C., Storhoff J. J., Nature, 1996, 382(6592), 607—609 |
[11] | Le J. D., Pinto Y., Seeman N. C., Musier-Forsyth K., Taton T. A., Kiehl R. A., Nano Lett., 2004, 4(12), 2343—2347 |
[12] | Deng Z. X., Tian Y., Lee S. H., Ribbe A. E., Mao C. D., Angew. Chem. Int. Ed., 2005, 44(23), 3582—3585 |
[13] | Cheglakov Z., Weizmann Y., Braunschweig A. B., Wilner O. I., Willner I., Angew. Chem. Int. Ed., 2008, 47(1), 126—130 |
[14] | Wilner O. I., Shimron S., Weizmann Y., Wang Z. G., Willner I., Nano Lett., 2009, 9(5), 2040—2043 |
[15] | Edwardson T. G., Carneiro K. M., McLaughlin C. K., Serpell C. J., Sleiman H. F., Nat. Chem., 2013, 5(10), 868—875 |
[16] | Zhang C., Li X., Tian C., Yu G., Li Y., Jiang W., Mao C. D., ACS Nano, 2014, 8(2), 1130—135 |
[17] | Yan H., Park S. H., Finkelstein G., Reif J. H., LaBean T. H., Science, 2003, 301(5641), 1882—1884 |
[18] | Ma X., Huh J., Park W., Lee L.P., Kwon Y. J., Sim S. J.,Nat. Comm., DOI: 10.1038/ncomms12873 |
[19] | Zanchet D., Micheel C. M., Parak W. J., Gerion D., Alivisatos A. P., Nano Lett., 2001, 1(1), 32—35 |
[20] | Ackerson C. J., Sykes M. T., Kornberg R. D., Proc. Natl. Acad. Sci. USA, 2005, 102(38), 13383—13385 |
[21] | Borovok N., Gillon E., Kotlyar A., Bioconjug. Chem., 2012, 23(5), 916—922 |
[22] | Ma N., Sargent E. H., Kelley S. O., Nat. Nanotechnol., 2009, 4(2), 121—125 |
[23] | Tikhomirov G., Hoogland S., Lee P. E., Fischer A., Sargent E. H., Kelley S. O., Nat. Nanotechnol., 2011, 6(8), 485—490 |
[24] | He X. W., Li Z., Chen M. Z., Ma N., Angew. Chem. Int. Ed., 2014, 53(52), 14447—14450 |
[25] | He X. W., Zeng T., Li Z., Wang G. L., Ma N., Angew. Chem. Int. Ed., 2016, 55(9), 3073—3076 |
[26] | Yu W., Lian H., Wen Z., Xiao G., Chem. Mater., 2003, 15(14), 2854—2860 |
[27] | Aldeek F., Safi M., Zhan N., Palui G., Mattoussi H., ACS Nano, 2013, 7(11), 10197—10210 |
[28] | Claridge S. A., Goh S. L., Frechet J. M., Williams S. C., Micheel C. M., Alivisatsos A. P., Chem. Mater., 2005, 17(7), 1638—1635 |
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