Direct Detection of Tetracycline in Honey by Neutral Desorption-Extractive Electrospray Ionization Mass Spectrometry†

doi:10.7503/cjcu20160127

Abstract

Abstract:

A novel platform of neutral desorption-extractive electrospray ionization mass spectrometry(ND-EESI-MS) for direct and rapid detection oftetracycline(TC) in honey sample without any sample pretreatment wa constructed. The results showed that the limit of detection was 1.08 ng/mL with a linear working range from 20 to 1000 ng/mL(R²>0.997) toward TC in honey; the recoveries and relative standard deviations of TC from spiked samples, at three fortification levels(50, 500, 1000 ng/mL), were 94.26%, 98.38%, 103.00% and 3.28%, 1.39%, 1.12%, respectively. Eight common commodity honey samples were detected by this method and the results reveal that 2 of them contain traces of TC, while TC is not found in the rest samples. HPLC was also applied for TC detection in the asme honey samples, and TC is not found. Therefore, this ND-EESI-MS method is sensitive, rapid and specific for quantitative analysis of TC in honey without any sample pretreatment.

Key words: Neutral desorption-extractive electrospray ionization, Mass spectrometry, Tetracycline, Honey, Direct analysis

CLC Number:

O652.1

TrendMD:

DENG Min,FANG Xiaowei,GUO Xiali,HUANG Xueyong,LIU Xingxing,YU Tenghui,LUO Liping. Direct Detection of Tetracycline in Honey by Neutral Desorption-Extractive Electrospray Ionization Mass Spectrometry^†[J]. Chem. J. Chinese Universities, 2016, 37(8): 1430.

Figures/Tables 4

Fig.1 ND-EESI-MS experimental device for TC detection in spiked honey

Fig.2 EESI-MS2(A) and ND-EESI-MS2(B) spectra of tetracycline (A) TC standard solution(500 ng/mL); (B) the spiked honey sample(500 ng/mL).

Fig.3 Calibration curve of tetracycline in spiked honey

Table 1 Comparison of tetracycline detection in spiked honey samples using ND-EESI-MS and HPLC

Method	Linear range/ (ng·mL^-1)	R²	LOD/ (ng·mL^-1)	Recovery of spiked honey(%)			RSD of spiked honey(%)
Method	Linear range/ (ng·mL^-1)	R²	LOD/ (ng·mL^-1)	50 ng/mL	500 ng/mL	1000 ng/mL	50 ng/mL	500 ng/mL	1000 ng/mL
ND-EESI-MS	20—1000	0.997	1.08	94.26	98.38	103.00	3.28	1.39	1.12
HPLC	50—1000	0.999	3.46	105.56	96.51	97.38	2.31	1.44	0.65

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