Biotransformation of Aconitum Alkaloids Before and After the Combination of Radix Aconiti Preparata by Rat Intestinal Flora Using Semiquantitative Analysis Method of Electrospray Ionization Mass Spectrometry

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (7): 1526.

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Biotransformation of Aconitum Alkaloids Before and After the Combination of Radix Aconiti Preparata by Rat Intestinal Flora Using Semiquantitative Analysis Method of Electrospray Ionization Mass Spectrometry

WANG Xi-Ye^1,2, PI Zi-Feng¹, LIU Wen-Long¹, SONG Feng-Rui¹, LIU Zhi-Qiang¹, LIU Shu-Ying^1*

1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Graduate School of the Chinese Academy of Science, Beijng 100049, China

Received:2010-09-13 Revised:2010-11-09 Online:2011-07-10 Published:2011-06-02
Contact: LIU Shu-Ying E-mail:syliu19@yahoo.com.cn
Supported by:
国家“九七三”计划中医理论专项基金(批准号: 2006CB504706)和国家自然科学基金(批准号: 81073040)资助.

Abstract

Abstract: The biotransformation of diester-diterpenoid alkaloids, monoester-diterpenoid alkaloids and lipo-alkaloids in the co-decoctions of Chinese medicine pairs was studied by electrospray ionization tandem mass spectrometry, and semiquantitativ ananlysis method of electrospray ionization tandem mass spectrometry was established for analysis of aconitum alkaloids before and after metabolism. The content changes of aconitum alkaloids with was analyzed under rat intestinal bacteria in the metabolic process of the co-decoctions of Radix Aconiti Preparata with Bulbus Fritillariae Thunbergii, Rhizoma Pinelliae Preparatum and Ampelopsis Japonica. The results showed that Bulbus Fritillariae Thunbergii increased the content of diester-diterpenoid alkaloids in co-decoction, Rhizoma Pinelliae Preparatum reduced the content of diester-diterpenoid alkaloids in co-decoction, and Ampelopsis Japonica almost has no effect on the content of diester-diterpenoid alkaloids in co-decoction, after the combination; The rat intestinal bacteria can more reduce the toxicity of diester-diterpenoid alkaloids and increase the content of lipo-alkaloids. The mechanism of combination and metabolism of traditional Chinese medicine was proved by the analysis of the content changes of aconitum alkaloids in the processes of combination and metabolism of Chinese medicine pairs

Key words: Radix Aconiti Preparata, Combination, Metabolism under Rat Intestinal Bacteria, Semiquantitativ ananlysis method of Electrospray ionization mass spectrometry

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WANG Xi-Ye, PI Zi-Feng, LIU Wen-Long, SONG Feng-Rui, LIU Zhi-Qiang, LIU Shu-Ying*. Biotransformation of Aconitum Alkaloids Before and After the Combination of Radix Aconiti Preparata by Rat Intestinal Flora Using Semiquantitative Analysis Method of Electrospray Ionization Mass Spectrometry[J]. Chem. J. Chinese Universities, 2011, 32(7): 1526.

References

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Biotransformation of Aconitum Alkaloids Before and After the Combination of Radix Aconiti Preparata by Rat Intestinal Flora Using Semiquantitative Analysis Method of Electrospray Ionization Mass Spectrometry

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