Oxygenation Reaction of Histidine Cobalt Complex by Electrospray Tandem Mass Spectrometry

Abstract

Abstract: Histidine-cobalt compex is a known model compound of oxygen carriers. The investigation of its oxygenation is of great significance in revealing the oxygenation mechanism of reversibly absorbing oxygen. In this paper, a new method was proposed to directly investigate the oxygenation reaction of histidine-cobalt compex by electrospray tandem mass spectrometry. The fragment ions of the oxygenated complexes were found from both aqueous samples of histidine-cobalt compex separately adsorbing oxygen at early 10-20 minutes and continuously adsorbing oxygen for 24 hours. The fragment ions of the oxygenated complexes were confirmed by its sub-fragment ion which just lost a neutral fragment of dioxygen by further fragmentating. Based on these results, the structure, mechanism and the form of dioxygen existed in the complex were deduced. The results showed that the absorption process is a dynamic process, and the structure of the complex could be changed from dinuclear superoxo-bridged complex to dinuclear superoxo-hydroxo dibridge complex according to the progress of oxygenation time. ESI-MS method can be an effective technique of researching the oxygenation reaction and characterizing oxygenated complexes

Key words: Histidine, Cobalt, ESI-MS/MS, Oxygenation, Oxygen carrier

CLC Number:

O657.6

TrendMD:

FU Ji-Hong, LI Jun-Fang, FU Pei, YUE Fan, ZHANG Xu-Long, WANG Ji-De*. Oxygenation Reaction of Histidine Cobalt Complex by Electrospray Tandem Mass Spectrometry[J]. Chem. J. Chinese Universities, 2011, 32(7): 1483.

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