Kinetics Study of Hydrolysis Catalyzed by Immobilized Enzyme in Nanozeolite Modified Microchannel Reactors

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (3): 753.

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Kinetics Study of Hydrolysis Catalyzed by Immobilized Enzyme in Nanozeolite Modified Microchannel Reactors

WANG Zhou-Jun, LI Xiang, WANG Chen-Chen, TANG Yi, ZHANG Ya-Hong*

Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200433, China

Received:2010-10-15 Revised:2010-12-17 Online:2011-03-10 Published:2011-02-23
Contact: ZHANG Ya-Hong E-mail:zhangyh@fudan.edu.cn
Supported by:
国家自然科学基金(批准号: 20873025)资助.

Abstract

Abstract: In this paper, nanozeolite modified microchannel reactors are constructed by assembling zeolite nanocrystals into capillary using layer-by-layer method. And then lipase is immobilized on the nanozeolites which are assembled in the microreactors. The nanozeolite modified microreactors could incorporate excellent biocaptibility and large external area of nanozeolites and high efficiency and fast mass diffusion of microreactors together. Hydrolysis of 4-nitrophenyl palmitate (4-NPP) is carried out as a probe reaction to study the hydrolysis kinetics catalyzed by immobilized lipase in the microreactors. By comparing the characteristic parameters (Km and Vmax) from Michaelis-Menten equation, it is found that efficiency of enzymatic hydrolysis in such nanozeolite modified microchannel reactors could be 3 or more times higher than that of the same reaction in conventional reactors. In addition affinity between enzyme and substrates is also enhanced in the nanozeolite modified microchannel reactors.

Key words: nanozeolite, microreactor, lipase, hydrolysis of 4-nitrophenyl palmitate

CLC Number:

O643

TrendMD:

WANG Zhou-Jun, LI Xiang, WANG Chen-Chen, TANG Yi, ZHANG Ya-Hong*. Kinetics Study of Hydrolysis Catalyzed by Immobilized Enzyme in Nanozeolite Modified Microchannel Reactors[J]. Chem. J. Chinese Universities, 2011, 32(3): 753.

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Kinetics Study of Hydrolysis Catalyzed by Immobilized Enzyme in Nanozeolite Modified Microchannel Reactors

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